Selected ion monitoring analysis of pseudoracemic hexobarbital and its major metabolites in blood and urine of rats

M van der Graaff, P H Hofman, D D Breimer, N P Vermeulen, J Knabe, L Schamber

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

A stereospecific synthesis of N1-(2H3)-labelled R(-)-hexobarbital is described. A sensitive and rapid selected ion monitoring assay procedure for pseudoracemic hexobarbital, consisting of equal amounts of S(+)-hexobarbital (1a) and (2H3)-R(-)-hexobarbital (1b) in 100 microliters blood samples of rats was developed. Both the parent enantiomers in blood and three major metabolites excreted in urine were quantified. An application of the method in rats is described, and the results are compared to previously obtained data of separately administered enantiomers.

Original languageEnglish
Pages (from-to)464-9
Number of pages6
JournalBiomedical mass spectrometry
Volume12
Issue number9
Publication statusPublished - Sep 1985

Keywords

  • Animals
  • Chromatography, Gas
  • Hexobarbital
  • Kinetics
  • Male
  • Rats
  • Rats, Inbred Strains
  • Stereoisomerism
  • Journal Article

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