TY - JOUR
T1 - Sensitive spectroscopic detection of large and denatured protein aggregates in solution by use of the fluorescent dye nile red
AU - Sutter, M.
AU - Oliveira, S.
AU - Sanders, N.N.
AU - Lucas, B.
AU - van Hoek, A.
AU - Hink, M.A.
AU - Visser, A.J.W.G.
AU - De Smedt, S.C.
AU - Hennink, W.E.
AU - Jiskoot, W.
PY - 2007
Y1 - 2007
N2 - The fluorescent dye Nile red was used as a probe for the sensitive detection of large, denatured aggregates of the model protein β-galactosidase (E. coli) in solution. Aggregates were formed by irreversible heat denaturation of β-galactosidase below and above the protein's unfolding temperature of 57.4°C, and the presence of aggregates in heated solutions was confirmed by static light scattering. Interaction of Nile red with β-galactosidase aggregates led to a shift of the emission maximum (λ
AB - The fluorescent dye Nile red was used as a probe for the sensitive detection of large, denatured aggregates of the model protein β-galactosidase (E. coli) in solution. Aggregates were formed by irreversible heat denaturation of β-galactosidase below and above the protein's unfolding temperature of 57.4°C, and the presence of aggregates in heated solutions was confirmed by static light scattering. Interaction of Nile red with β-galactosidase aggregates led to a shift of the emission maximum (λ
UR - https://www.scopus.com/pages/publications/33847608685
UR - https://www.scopus.com/inward/citedby.url?scp=33847608685&partnerID=8YFLogxK
U2 - 10.1007/s10895-007-0156-6
DO - 10.1007/s10895-007-0156-6
M3 - Article
SN - 1053-0509
VL - 17
SP - 181
EP - 192
JO - Journal of Fluorescence
JF - Journal of Fluorescence
ER -
