Abstract
Subjects. Apparently 124 subjects were recruited from clinics at the university dental school. However, the total number of subjects used in the study is unclear; Table 1 reports a total of 112 subjects and Table 2 reports smoking data for 122. Table 2 reports a nearly equal distribution of males/females (49% versus 51%). There is no information on the dates/period of recruitment. There is no flow chart presented on numbers of subjects screened, or subjects excluded or refusing to participate. All dental patients could be included as far as has been reported, except those with “systemic disease that could affect neutrophil function.” However, individuals with diabetes, and perhaps rheumatoid arthritis and other diseases, could be included. Moreover “neutrophil function” is not studied here; only neutrophil counts.
Key Risk/Study Factor. In this report “periodontitis” is identified as the cause for increased numbers of oPMN leukocytes in an oral rinse sample.
Main Outcome Measure. The main outcome measure is total number (count) of oPMN leukocytes in an oral rinse sample.
Main Results. Patients with severe periodontitis had 5.5 × 106 (standard error 6.4 × 106) oPMN leukocytes from one oral rinse with 10 ml PBS for 30 s, compared with 0.7 × 106 (standard error 0.1 × 106) in individuals with a healthy periodontium (also not having gingivitis). Moreover, there were correlations between oPMN numbers and numbers of pockets >5 mm and % of sites with bleeding on probing. These results have not been adjusted for potential confounding factors, such as sex, age, diabetes, or smoking. Only non-parametric statistics have been used. Of note, the numbers of oPMN leukocytes are derived from 10 ml rinse, but after centrifugation resuspended in 5 ml PBS; the reported numbers therefore are twice as high as in the original 10-ml rinse sample, if one considers a “per ml” unit.
Conclusions. The authors show increased numbers of oPMN leukocytes in an oral rinse with 10 ml PBS of 30 s in patients with severe periodontitis compared with periodontally healthy subjects. There were no significant differences found for oPMN numbers between healthy on one side and mild or moderate periodontitis or gingivitis on the other side.
Key Risk/Study Factor. In this report “periodontitis” is identified as the cause for increased numbers of oPMN leukocytes in an oral rinse sample.
Main Outcome Measure. The main outcome measure is total number (count) of oPMN leukocytes in an oral rinse sample.
Main Results. Patients with severe periodontitis had 5.5 × 106 (standard error 6.4 × 106) oPMN leukocytes from one oral rinse with 10 ml PBS for 30 s, compared with 0.7 × 106 (standard error 0.1 × 106) in individuals with a healthy periodontium (also not having gingivitis). Moreover, there were correlations between oPMN numbers and numbers of pockets >5 mm and % of sites with bleeding on probing. These results have not been adjusted for potential confounding factors, such as sex, age, diabetes, or smoking. Only non-parametric statistics have been used. Of note, the numbers of oPMN leukocytes are derived from 10 ml rinse, but after centrifugation resuspended in 5 ml PBS; the reported numbers therefore are twice as high as in the original 10-ml rinse sample, if one considers a “per ml” unit.
Conclusions. The authors show increased numbers of oPMN leukocytes in an oral rinse with 10 ml PBS of 30 s in patients with severe periodontitis compared with periodontally healthy subjects. There were no significant differences found for oPMN numbers between healthy on one side and mild or moderate periodontitis or gingivitis on the other side.
| Original language | English |
|---|---|
| Pages (from-to) | 73-74 |
| Journal | The Journal of Evidence-Based Dental Practice |
| Volume | 16 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Mar 2016 |
Bibliographical note
Article review:Quantifying oral inflammatory load: oral neutrophil counts in periodontal health and disease. Landzberg M, Doering H, Aboodi GM, Tenenbaum HC, Glogauer M. J Periodontal Res 2015;50(3):330-6.
