Sheltering behavior and locomotor activity in 11 genetically diverse common inbred mouse strains using home-cage monitoring

M. Loos, B. Koopmans, E. Aarts, G.P. Maroteaux, S. van der Sluis, M. Verhage, A.B. Smit

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Functional genetic analyses in mice rely on efficient and in-depth characterization of the behavioral spectrum. Automated home-cage observation can provide a systematic and efficient screening method to detect unexplored, novel behavioral phenotypes. Here, we analyzed high-throughput automated home-cage data using existing and novel concepts, to detect a plethora of genetic differences in spontaneous behavior in a panel of commonly used inbred strains (129S1/SvImJ, A/J, C3H/HeJ, C57BL/6J, BALB/cJ, DBA/2J, NOD/LtJ, FVB/NJ, WSB/EiJ, PWK/PhJ and CAST/EiJ). Continuous video-tracking observations of sheltering behavior and locomotor activity were segmented into distinguishable behavioral elements, and studied at different time scales, yielding a set of 115 behavioral parameters of which 105 showed highly significant strain differences. This set of 115 parameters was highly dimensional; principal component analysis identified 26 orthogonal components with eigenvalues above one. Especially novel parameters of sheltering behavior and parameters describing aspects of motion of the mouse in the home-cage showed high genetic effect sizes. Multi-day habituation curves and patterns of behavior surrounding dark/light phase transitions showed striking strain differences, albeit with lower genetic effect sizes. This spontaneous home-cage behavior study demonstrates high dimensionality, with a strong genetic contribution to specific sets of behavioral measures. Importantly, spontaneous home-cage behavior analysis detects genetic effects that cannot be studied in conventional behavioral tests, showing that the inclusion of a few days of undisturbed, labor extensive homecage assessment may greatly aid gene function analyses and drug target discovery.
Original languageEnglish
Article numbere108563
Pages (from-to)e108563
JournalPLoS ONE
Volume9
Issue number9
DOIs
Publication statusPublished - 2014

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Inbred Strains Mice
Locomotion
locomotion
cages
Monitoring
monitoring
mice
strain differences
Principal component analysis
Screening
Genes
Phase transitions
Throughput
Personnel
Phase Transition
Drug Discovery
Principal Component Analysis
phase transition
Pharmaceutical Preparations
genetic techniques and protocols

Cite this

@article{28731bad27dd492c89f1064be9309dda,
title = "Sheltering behavior and locomotor activity in 11 genetically diverse common inbred mouse strains using home-cage monitoring",
abstract = "Functional genetic analyses in mice rely on efficient and in-depth characterization of the behavioral spectrum. Automated home-cage observation can provide a systematic and efficient screening method to detect unexplored, novel behavioral phenotypes. Here, we analyzed high-throughput automated home-cage data using existing and novel concepts, to detect a plethora of genetic differences in spontaneous behavior in a panel of commonly used inbred strains (129S1/SvImJ, A/J, C3H/HeJ, C57BL/6J, BALB/cJ, DBA/2J, NOD/LtJ, FVB/NJ, WSB/EiJ, PWK/PhJ and CAST/EiJ). Continuous video-tracking observations of sheltering behavior and locomotor activity were segmented into distinguishable behavioral elements, and studied at different time scales, yielding a set of 115 behavioral parameters of which 105 showed highly significant strain differences. This set of 115 parameters was highly dimensional; principal component analysis identified 26 orthogonal components with eigenvalues above one. Especially novel parameters of sheltering behavior and parameters describing aspects of motion of the mouse in the home-cage showed high genetic effect sizes. Multi-day habituation curves and patterns of behavior surrounding dark/light phase transitions showed striking strain differences, albeit with lower genetic effect sizes. This spontaneous home-cage behavior study demonstrates high dimensionality, with a strong genetic contribution to specific sets of behavioral measures. Importantly, spontaneous home-cage behavior analysis detects genetic effects that cannot be studied in conventional behavioral tests, showing that the inclusion of a few days of undisturbed, labor extensive homecage assessment may greatly aid gene function analyses and drug target discovery.",
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Sheltering behavior and locomotor activity in 11 genetically diverse common inbred mouse strains using home-cage monitoring. / Loos, M.; Koopmans, B.; Aarts, E.; Maroteaux, G.P.; van der Sluis, S.; Verhage, M.; Smit, A.B.

In: PLoS ONE, Vol. 9, No. 9, e108563, 2014, p. e108563.

Research output: Contribution to JournalArticleAcademicpeer-review

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AU - Loos, M.

AU - Koopmans, B.

AU - Aarts, E.

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AU - van der Sluis, S.

AU - Verhage, M.

AU - Smit, A.B.

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