Short-coherence off-axis holographic phase microscopy of live cell dynamics

S. Witte, A. Plauska, M.C. Ridder, L. van Berge, H.D. Mansvelder, M.L. Groot

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We demonstrate a single-shot holographic phase microscope that combines short-coherence laser pulses with an off-axis geometry. By introducing a controlled pulse front tilt, ultrashort pulses are made to interfere over a large field-of-view without loss of fringe contrast. With this microscope, quantitative phase images of live cells can be recorded in a fullfield geometry without moving parts. We perform phase imaging of HEK293 cells, to study the dynamics of cell volume regulation in response to an osmotic shock. © 2012 Optical Society of America.
Original languageEnglish
Pages (from-to)2184-2189
Number of pages5
JournalBiomedical Optics Express
Issue number9
Publication statusPublished - 2012


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