Abstract
We demonstrate a single-shot holographic phase microscope that combines short-coherence laser pulses with an off-axis geometry. By introducing a controlled pulse front tilt, ultrashort pulses are made to interfere over a large field-of-view without loss of fringe contrast. With this microscope, quantitative phase images of live cells can be recorded in a fullfield geometry without moving parts. We perform phase imaging of HEK293 cells, to study the dynamics of cell volume regulation in response to an osmotic shock. © 2012 Optical Society of America.
| Original language | English |
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| Pages (from-to) | 2184-2189 |
| Number of pages | 5 |
| Journal | Biomedical Optics Express |
| Volume | 3 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2012 |