High Performance Liquid Chromatography combined with fluorescence detection is routinely used in the Dutch Water Quality Survey to determine the Polycyclic Aromatic Hydrocarbons (PAH) contents of marine sediment samples. In this paper Shpol'skii spectroscopy is utilized to ascertain the identity and the purity of the peaks in the chromatogram by collection of several eluting fractions and subsequent spectroscopic analysis. The low temperature Shpol'skii technique provides high-resolution fluorescence spectra of PAHs that can serve as fingerprints. Thus, information concerning peak purity was obtained and the number of components identified was roughly doubled.
Mastenbroek, J. W. G., ARIESE, F., GOOIJER, C., Velthorst, N. H., Hofstraat, J. W., & VANZEIJL, WJM. (1990). SHPOLSKII FLUOROMETRY AS AN INDEPENDENT IDENTIFICATION METHOD TO UPGRADE ROUTINE HPLC ANALYSIS OF POLYCYCLIC AROMATIC-HYDROCARBONS. Chemosphere, 21(3), 377-386. https://doi.org/10.1016/0045-6535(90)90007-G