Sialylation of Campylobacter jejuni lipo-oligosaccharides is associated with severe gastro-enteritis and reactive arthritis

N.P. Mortensen, M.L. Kuijf, C.W. Ang, P. Schiellerup, K.A. Krogfelt, B.F. Jacobs, A. van Belkum, H.Ph. Endtz, M.P. Bergman

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

We used various genotyping methods to identify bacterial genetic markers for development of arthritic symptoms following Campylobacter enteritis. We genotyped a collection of population derived Campylobacter strains, with detailed information on clinical characteristics, including arthritic symptoms. Besides using whole genome screening methods, we focused on the lipo-oligosaccharide (LOS) gene locus in which marker genes for developing post-Campylobacter neurological disease are present. Patients with arthritic symptoms were more frequently infected with Campylobacter jejuni strains with a class A LOS locus. We also found that patients who were infected with a C. jejuni strain containing sialic acid-positive LOS (class A, B or C) more frequently had bloody diarrhoea and a longer duration of symptoms. Furthermore, the IgM antibody response against Campylobacter was stronger in patients with a sialic acid containing LOS. Ganglioside auto-antibodies were observed in a small number of patients following infection with a class C strain. We conclude that sialylation of C. jejuni LOS is not only a risk factor for development of post-infectious symptoms, but is also associated with increased severity of enteric disease. © 2009 Elsevier Masson SAS. All rights reserved.
Original languageEnglish
Pages (from-to)988-994
JournalMicrobes and Infection
Volume11
Issue number12
DOIs
Publication statusPublished - 2009

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Reactive Arthritis
Campylobacter jejuni
Enteritis
Oligosaccharides
Campylobacter
Arthritis
N-Acetylneuraminic Acid
Gangliosides
Genetic Markers
Genes
Antibody Formation
Immunoglobulin M
Diarrhea
Genome
Antibodies
Infection
Population

Bibliographical note

J OCT

Cite this

Mortensen, N.P. ; Kuijf, M.L. ; Ang, C.W. ; Schiellerup, P. ; Krogfelt, K.A. ; Jacobs, B.F. ; van Belkum, A. ; Endtz, H.Ph. ; Bergman, M.P. / Sialylation of Campylobacter jejuni lipo-oligosaccharides is associated with severe gastro-enteritis and reactive arthritis. In: Microbes and Infection. 2009 ; Vol. 11, No. 12. pp. 988-994.
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abstract = "We used various genotyping methods to identify bacterial genetic markers for development of arthritic symptoms following Campylobacter enteritis. We genotyped a collection of population derived Campylobacter strains, with detailed information on clinical characteristics, including arthritic symptoms. Besides using whole genome screening methods, we focused on the lipo-oligosaccharide (LOS) gene locus in which marker genes for developing post-Campylobacter neurological disease are present. Patients with arthritic symptoms were more frequently infected with Campylobacter jejuni strains with a class A LOS locus. We also found that patients who were infected with a C. jejuni strain containing sialic acid-positive LOS (class A, B or C) more frequently had bloody diarrhoea and a longer duration of symptoms. Furthermore, the IgM antibody response against Campylobacter was stronger in patients with a sialic acid containing LOS. Ganglioside auto-antibodies were observed in a small number of patients following infection with a class C strain. We conclude that sialylation of C. jejuni LOS is not only a risk factor for development of post-infectious symptoms, but is also associated with increased severity of enteric disease. {\circledC} 2009 Elsevier Masson SAS. All rights reserved.",
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Mortensen, NP, Kuijf, ML, Ang, CW, Schiellerup, P, Krogfelt, KA, Jacobs, BF, van Belkum, A, Endtz, HP & Bergman, MP 2009, 'Sialylation of Campylobacter jejuni lipo-oligosaccharides is associated with severe gastro-enteritis and reactive arthritis' Microbes and Infection, vol. 11, no. 12, pp. 988-994. https://doi.org/10.1016/j.micinf.2009.07.004

Sialylation of Campylobacter jejuni lipo-oligosaccharides is associated with severe gastro-enteritis and reactive arthritis. / Mortensen, N.P.; Kuijf, M.L.; Ang, C.W.; Schiellerup, P.; Krogfelt, K.A.; Jacobs, B.F.; van Belkum, A.; Endtz, H.Ph.; Bergman, M.P.

In: Microbes and Infection, Vol. 11, No. 12, 2009, p. 988-994.

Research output: Contribution to JournalArticleAcademicpeer-review

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T1 - Sialylation of Campylobacter jejuni lipo-oligosaccharides is associated with severe gastro-enteritis and reactive arthritis

AU - Mortensen, N.P.

AU - Kuijf, M.L.

AU - Ang, C.W.

AU - Schiellerup, P.

AU - Krogfelt, K.A.

AU - Jacobs, B.F.

AU - van Belkum, A.

AU - Endtz, H.Ph.

AU - Bergman, M.P.

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N2 - We used various genotyping methods to identify bacterial genetic markers for development of arthritic symptoms following Campylobacter enteritis. We genotyped a collection of population derived Campylobacter strains, with detailed information on clinical characteristics, including arthritic symptoms. Besides using whole genome screening methods, we focused on the lipo-oligosaccharide (LOS) gene locus in which marker genes for developing post-Campylobacter neurological disease are present. Patients with arthritic symptoms were more frequently infected with Campylobacter jejuni strains with a class A LOS locus. We also found that patients who were infected with a C. jejuni strain containing sialic acid-positive LOS (class A, B or C) more frequently had bloody diarrhoea and a longer duration of symptoms. Furthermore, the IgM antibody response against Campylobacter was stronger in patients with a sialic acid containing LOS. Ganglioside auto-antibodies were observed in a small number of patients following infection with a class C strain. We conclude that sialylation of C. jejuni LOS is not only a risk factor for development of post-infectious symptoms, but is also associated with increased severity of enteric disease. © 2009 Elsevier Masson SAS. All rights reserved.

AB - We used various genotyping methods to identify bacterial genetic markers for development of arthritic symptoms following Campylobacter enteritis. We genotyped a collection of population derived Campylobacter strains, with detailed information on clinical characteristics, including arthritic symptoms. Besides using whole genome screening methods, we focused on the lipo-oligosaccharide (LOS) gene locus in which marker genes for developing post-Campylobacter neurological disease are present. Patients with arthritic symptoms were more frequently infected with Campylobacter jejuni strains with a class A LOS locus. We also found that patients who were infected with a C. jejuni strain containing sialic acid-positive LOS (class A, B or C) more frequently had bloody diarrhoea and a longer duration of symptoms. Furthermore, the IgM antibody response against Campylobacter was stronger in patients with a sialic acid containing LOS. Ganglioside auto-antibodies were observed in a small number of patients following infection with a class C strain. We conclude that sialylation of C. jejuni LOS is not only a risk factor for development of post-infectious symptoms, but is also associated with increased severity of enteric disease. © 2009 Elsevier Masson SAS. All rights reserved.

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DO - 10.1016/j.micinf.2009.07.004

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JF - Microbes and Infection

SN - 1286-4579

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ER -