Significant salivary changes in relation to oral mucositis following autologous hematopoietic stem cell transplantation

S.J.M. van Leeuwen; G.B. Proctor; A.M.G.A. Laheij; C.M.J. Potting; O. Smits; E.M. Bronkhorst; M.D. Hazenberg; T.M. Haverman; M.T. Brennan; I. von Bültzingslöwen; J.E. Raber-Durlacher; M.C.D.N.J.M. Huysmans; F.R. Rozema; N.M.A. Blijlevens

doi:10.1038/s41409-020-01185-7

Significant salivary changes in relation to oral mucositis following autologous hematopoietic stem cell transplantation

S.J.M. van Leeuwen, G.B. Proctor, A.M.G.A. Laheij, C.M.J. Potting, O. Smits, E.M. Bronkhorst, M.D. Hazenberg, T.M. Haverman, M.T. Brennan, I. von Bültzingslöwen, J.E. Raber-Durlacher, M.C.D.N.J.M. Huysmans, F.R. Rozema, N.M.A. Blijlevens

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

© 2020, The Author(s).The aim of this multicentre, longitudinal study was to determine salivary changes in relation to oral mucositis (OM) in multiple myeloma patients following high-dose melphalan and autologous hematopoietic stem cell transplantation (ASCT). Unstimulated and stimulated whole-mouth saliva samples (UWS and SWS) were collected before ASCT, 1×/wk during the hospitalisation phase, and 3 and 12 months post-ASCT. During the hospitalisation period OM was scored 3×/wk (WHO system). Flow rate, pH, total protein concentration (Nanodrop), albumin, lactoferrin, neutrophil defensin-1 (HNP1), total IgA and S100A8/A9 (ELISA) were determined. Mixed models were used to evaluate differences between ulcerative (u)OM (≥2 WHO, n = 20) and non-uOM (n = 31) groups. Until 18 days after ASCT, flow rate, pH, total IgA and HNP1 levels decreased in UWS and/or SWS, while log lactoferrin levels were significantly increased (UWS: p = 0.016 95% CI [0.36, 3.58], SWS: p < 0.001 95% CI [1.14, 3.29]). Twelve months post-ASCT, salivary protein levels were similar to baseline except for log total IgA, which was higher (UWS: p < 0.001 95% CI [0.49, 1.29], SWS: p < 0.001 95% CI [0.72, 1.45]). No differences between uOM and non-uOM groups were observed. Changes in salivary proteins indicated an inflammatory reaction in salivary glands coinciding with mucosal and systemic reactions in response to high-dose melphalan.

Original language	English
Pages (from-to)	1381-1390
Journal	Bone Marrow Transplantation
Volume	56
Issue number	6
DOIs	https://doi.org/10.1038/s41409-020-01185-7
Publication status	Published - 1 Jun 2021

Funding

Acknowledgements The study was funded by the Dutch Cancer Society (ACTA 2014-7468). For the help with the laboratory analysis of the total IgA levels in supernatant and precipitate we want to thank M.A.C. Sanders and M. Struik. For the help with the data collection we want to thank R.G.A. Koppelmans. This study was funded by: Dutch Cancer Society.

Funders	Funder number
Dutch Cancer Society	ACTA 2014-7468

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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van Leeuwen, S. J. M., Proctor, G. B., Laheij, A. M. G. A., Potting, C. M. J., Smits, O., Bronkhorst, E. M., Hazenberg, M. D., Haverman, T. M., Brennan, M. T., von Bültzingslöwen, I., Raber-Durlacher, J. E., Huysmans, M. C. D. N. J. M., Rozema, F. R., & Blijlevens, N. M. A. (2021). Significant salivary changes in relation to oral mucositis following autologous hematopoietic stem cell transplantation. Bone Marrow Transplantation, 56(6), 1381-1390. https://doi.org/10.1038/s41409-020-01185-7

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title = "Significant salivary changes in relation to oral mucositis following autologous hematopoietic stem cell transplantation",

abstract = "{\textcopyright} 2020, The Author(s).The aim of this multicentre, longitudinal study was to determine salivary changes in relation to oral mucositis (OM) in multiple myeloma patients following high-dose melphalan and autologous hematopoietic stem cell transplantation (ASCT). Unstimulated and stimulated whole-mouth saliva samples (UWS and SWS) were collected before ASCT, 1×/wk during the hospitalisation phase, and 3 and 12 months post-ASCT. During the hospitalisation period OM was scored 3×/wk (WHO system). Flow rate, pH, total protein concentration (Nanodrop), albumin, lactoferrin, neutrophil defensin-1 (HNP1), total IgA and S100A8/A9 (ELISA) were determined. Mixed models were used to evaluate differences between ulcerative (u)OM (≥2 WHO, n = 20) and non-uOM (n = 31) groups. Until 18 days after ASCT, flow rate, pH, total IgA and HNP1 levels decreased in UWS and/or SWS, while log lactoferrin levels were significantly increased (UWS: p = 0.016 95% CI [0.36, 3.58], SWS: p < 0.001 95% CI [1.14, 3.29]). Twelve months post-ASCT, salivary protein levels were similar to baseline except for log total IgA, which was higher (UWS: p < 0.001 95% CI [0.49, 1.29], SWS: p < 0.001 95% CI [0.72, 1.45]). No differences between uOM and non-uOM groups were observed. Changes in salivary proteins indicated an inflammatory reaction in salivary glands coinciding with mucosal and systemic reactions in response to high-dose melphalan.",

author = "{van Leeuwen}, S.J.M. and G.B. Proctor and A.M.G.A. Laheij and C.M.J. Potting and O. Smits and E.M. Bronkhorst and M.D. Hazenberg and T.M. Haverman and M.T. Brennan and {von B{\"u}ltzingsl{\"o}wen}, I. and J.E. Raber-Durlacher and M.C.D.N.J.M. Huysmans and F.R. Rozema and N.M.A. Blijlevens",

year = "2021",

month = jun,

day = "1",

doi = "10.1038/s41409-020-01185-7",

language = "English",

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van Leeuwen, SJM, Proctor, GB, Laheij, AMGA, Potting, CMJ, Smits, O, Bronkhorst, EM, Hazenberg, MD, Haverman, TM, Brennan, MT, von Bültzingslöwen, I, Raber-Durlacher, JE, Huysmans, MCDNJM, Rozema, FR & Blijlevens, NMA 2021, 'Significant salivary changes in relation to oral mucositis following autologous hematopoietic stem cell transplantation', Bone Marrow Transplantation, vol. 56, no. 6, pp. 1381-1390. https://doi.org/10.1038/s41409-020-01185-7

TY - JOUR

T1 - Significant salivary changes in relation to oral mucositis following autologous hematopoietic stem cell transplantation

AU - van Leeuwen, S.J.M.

AU - Proctor, G.B.

AU - Laheij, A.M.G.A.

AU - Potting, C.M.J.

AU - Smits, O.

AU - Bronkhorst, E.M.

AU - Hazenberg, M.D.

AU - Haverman, T.M.

AU - Brennan, M.T.

AU - von Bültzingslöwen, I.

AU - Raber-Durlacher, J.E.

AU - Huysmans, M.C.D.N.J.M.

AU - Rozema, F.R.

AU - Blijlevens, N.M.A.

PY - 2021/6/1

Y1 - 2021/6/1

N2 - © 2020, The Author(s).The aim of this multicentre, longitudinal study was to determine salivary changes in relation to oral mucositis (OM) in multiple myeloma patients following high-dose melphalan and autologous hematopoietic stem cell transplantation (ASCT). Unstimulated and stimulated whole-mouth saliva samples (UWS and SWS) were collected before ASCT, 1×/wk during the hospitalisation phase, and 3 and 12 months post-ASCT. During the hospitalisation period OM was scored 3×/wk (WHO system). Flow rate, pH, total protein concentration (Nanodrop), albumin, lactoferrin, neutrophil defensin-1 (HNP1), total IgA and S100A8/A9 (ELISA) were determined. Mixed models were used to evaluate differences between ulcerative (u)OM (≥2 WHO, n = 20) and non-uOM (n = 31) groups. Until 18 days after ASCT, flow rate, pH, total IgA and HNP1 levels decreased in UWS and/or SWS, while log lactoferrin levels were significantly increased (UWS: p = 0.016 95% CI [0.36, 3.58], SWS: p < 0.001 95% CI [1.14, 3.29]). Twelve months post-ASCT, salivary protein levels were similar to baseline except for log total IgA, which was higher (UWS: p < 0.001 95% CI [0.49, 1.29], SWS: p < 0.001 95% CI [0.72, 1.45]). No differences between uOM and non-uOM groups were observed. Changes in salivary proteins indicated an inflammatory reaction in salivary glands coinciding with mucosal and systemic reactions in response to high-dose melphalan.

AB - © 2020, The Author(s).The aim of this multicentre, longitudinal study was to determine salivary changes in relation to oral mucositis (OM) in multiple myeloma patients following high-dose melphalan and autologous hematopoietic stem cell transplantation (ASCT). Unstimulated and stimulated whole-mouth saliva samples (UWS and SWS) were collected before ASCT, 1×/wk during the hospitalisation phase, and 3 and 12 months post-ASCT. During the hospitalisation period OM was scored 3×/wk (WHO system). Flow rate, pH, total protein concentration (Nanodrop), albumin, lactoferrin, neutrophil defensin-1 (HNP1), total IgA and S100A8/A9 (ELISA) were determined. Mixed models were used to evaluate differences between ulcerative (u)OM (≥2 WHO, n = 20) and non-uOM (n = 31) groups. Until 18 days after ASCT, flow rate, pH, total IgA and HNP1 levels decreased in UWS and/or SWS, while log lactoferrin levels were significantly increased (UWS: p = 0.016 95% CI [0.36, 3.58], SWS: p < 0.001 95% CI [1.14, 3.29]). Twelve months post-ASCT, salivary protein levels were similar to baseline except for log total IgA, which was higher (UWS: p < 0.001 95% CI [0.49, 1.29], SWS: p < 0.001 95% CI [0.72, 1.45]). No differences between uOM and non-uOM groups were observed. Changes in salivary proteins indicated an inflammatory reaction in salivary glands coinciding with mucosal and systemic reactions in response to high-dose melphalan.

U2 - 10.1038/s41409-020-01185-7

DO - 10.1038/s41409-020-01185-7

M3 - Article

SN - 0268-3369

VL - 56

SP - 1381

EP - 1390

JO - Bone Marrow Transplantation

JF - Bone Marrow Transplantation

IS - 6

ER -

Significant salivary changes in relation to oral mucositis following autologous hematopoietic stem cell transplantation

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