Simultaneous determination of blood concentrations of methohexital and its hydroxy metabolite by gas chromatography and identification of 4'-hydroxymethohexital by combined gas--liquid chromatography--mass spectrometry

H Heusler, J.D. Epping, S Heusler, E.A. Richter, N P Vermeulen, D D Breimer

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

A simple, sensitive and selective method is described for the simultaneous determination of low concentrations (less than 50 ng/ml) of underivatized methohexital and its hydroxy metabolite in small (0.1 ml) samples of human and rat plasma or whole blood by gas chromatography with nitrogen-selective detection. Moreover, the main metabolite in rat and man was identified as 4'-hydroxymethohexital by comparison of chromatograms from gas--liquid chromatography (GLC) with data obtained from GLC--mass spectrometry and 1H-nuclear magnetic resonance spectrometry of this metabolite, produced both by incubating methohexital with isolated rat liver microsomes and by isolating this metabolite from rat urine.

Original languageEnglish
Pages (from-to)403-12
Number of pages10
JournalJournal of Chromatography A
Volume226
Issue number2
Publication statusPublished - 11 Dec 1981

Fingerprint

Methohexital
Liquid chromatography
Metabolites
Gas chromatography
Gas Chromatography
Gas Chromatography-Mass Spectrometry
Mass spectrometry
Rats
Blood
Liver Microsomes
Liver
Spectrometry
Spectrum Analysis
Magnetic Resonance Spectroscopy
Nitrogen
Nuclear magnetic resonance
Urine
Plasmas
4'-hydroxymethohexital

Keywords

  • Animals
  • Chromatography, Gas
  • Gas Chromatography-Mass Spectrometry
  • Humans
  • Magnetic Resonance Spectroscopy
  • Methohexital
  • Microsomes, Liver
  • Rats
  • Comparative Study
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

@article{1f07c91373b94389beddb05083932cc6,
title = "Simultaneous determination of blood concentrations of methohexital and its hydroxy metabolite by gas chromatography and identification of 4'-hydroxymethohexital by combined gas--liquid chromatography--mass spectrometry",
abstract = "A simple, sensitive and selective method is described for the simultaneous determination of low concentrations (less than 50 ng/ml) of underivatized methohexital and its hydroxy metabolite in small (0.1 ml) samples of human and rat plasma or whole blood by gas chromatography with nitrogen-selective detection. Moreover, the main metabolite in rat and man was identified as 4'-hydroxymethohexital by comparison of chromatograms from gas--liquid chromatography (GLC) with data obtained from GLC--mass spectrometry and 1H-nuclear magnetic resonance spectrometry of this metabolite, produced both by incubating methohexital with isolated rat liver microsomes and by isolating this metabolite from rat urine.",
keywords = "Animals, Chromatography, Gas, Gas Chromatography-Mass Spectrometry, Humans, Magnetic Resonance Spectroscopy, Methohexital, Microsomes, Liver, Rats, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't",
author = "H Heusler and J.D. Epping and S Heusler and E.A. Richter and Vermeulen, {N P} and Breimer, {D D}",
year = "1981",
month = "12",
day = "11",
language = "English",
volume = "226",
pages = "403--12",
journal = "Journal of Chromatography A",
issn = "0021-9673",
publisher = "Elsevier",
number = "2",

}

Simultaneous determination of blood concentrations of methohexital and its hydroxy metabolite by gas chromatography and identification of 4'-hydroxymethohexital by combined gas--liquid chromatography--mass spectrometry. / Heusler, H; Epping, J.D.; Heusler, S; Richter, E.A.; Vermeulen, N P; Breimer, D D.

In: Journal of Chromatography A, Vol. 226, No. 2, 11.12.1981, p. 403-12.

Research output: Contribution to JournalArticleAcademicpeer-review

TY - JOUR

T1 - Simultaneous determination of blood concentrations of methohexital and its hydroxy metabolite by gas chromatography and identification of 4'-hydroxymethohexital by combined gas--liquid chromatography--mass spectrometry

AU - Heusler, H

AU - Epping, J.D.

AU - Heusler, S

AU - Richter, E.A.

AU - Vermeulen, N P

AU - Breimer, D D

PY - 1981/12/11

Y1 - 1981/12/11

N2 - A simple, sensitive and selective method is described for the simultaneous determination of low concentrations (less than 50 ng/ml) of underivatized methohexital and its hydroxy metabolite in small (0.1 ml) samples of human and rat plasma or whole blood by gas chromatography with nitrogen-selective detection. Moreover, the main metabolite in rat and man was identified as 4'-hydroxymethohexital by comparison of chromatograms from gas--liquid chromatography (GLC) with data obtained from GLC--mass spectrometry and 1H-nuclear magnetic resonance spectrometry of this metabolite, produced both by incubating methohexital with isolated rat liver microsomes and by isolating this metabolite from rat urine.

AB - A simple, sensitive and selective method is described for the simultaneous determination of low concentrations (less than 50 ng/ml) of underivatized methohexital and its hydroxy metabolite in small (0.1 ml) samples of human and rat plasma or whole blood by gas chromatography with nitrogen-selective detection. Moreover, the main metabolite in rat and man was identified as 4'-hydroxymethohexital by comparison of chromatograms from gas--liquid chromatography (GLC) with data obtained from GLC--mass spectrometry and 1H-nuclear magnetic resonance spectrometry of this metabolite, produced both by incubating methohexital with isolated rat liver microsomes and by isolating this metabolite from rat urine.

KW - Animals

KW - Chromatography, Gas

KW - Gas Chromatography-Mass Spectrometry

KW - Humans

KW - Magnetic Resonance Spectroscopy

KW - Methohexital

KW - Microsomes, Liver

KW - Rats

KW - Comparative Study

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - Article

VL - 226

SP - 403

EP - 412

JO - Journal of Chromatography A

JF - Journal of Chromatography A

SN - 0021-9673

IS - 2

ER -