Simultaneous determination of blood concentrations of methohexital and its hydroxy metabolite by gas chromatography and identification of 4'-hydroxymethohexital by combined gas--liquid chromatography--mass spectrometry

H Heusler, J.D. Epping, S Heusler, E.A. Richter, N P Vermeulen, D D Breimer

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

A simple, sensitive and selective method is described for the simultaneous determination of low concentrations (less than 50 ng/ml) of underivatized methohexital and its hydroxy metabolite in small (0.1 ml) samples of human and rat plasma or whole blood by gas chromatography with nitrogen-selective detection. Moreover, the main metabolite in rat and man was identified as 4'-hydroxymethohexital by comparison of chromatograms from gas--liquid chromatography (GLC) with data obtained from GLC--mass spectrometry and 1H-nuclear magnetic resonance spectrometry of this metabolite, produced both by incubating methohexital with isolated rat liver microsomes and by isolating this metabolite from rat urine.

Original languageEnglish
Pages (from-to)403-12
Number of pages10
JournalJournal of Chromatography A
Volume226
Issue number2
Publication statusPublished - 11 Dec 1981

Keywords

  • Animals
  • Chromatography, Gas
  • Gas Chromatography-Mass Spectrometry
  • Humans
  • Magnetic Resonance Spectroscopy
  • Methohexital
  • Microsomes, Liver
  • Rats
  • Comparative Study
  • Journal Article
  • Research Support, Non-U.S. Gov't

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