Abstract
A simple, sensitive and selective method is described for the simultaneous determination of low concentrations (less than 50 ng/ml) of underivatized methohexital and its hydroxy metabolite in small (0.1 ml) samples of human and rat plasma or whole blood by gas chromatography with nitrogen-selective detection. Moreover, the main metabolite in rat and man was identified as 4'-hydroxymethohexital by comparison of chromatograms from gas--liquid chromatography (GLC) with data obtained from GLC--mass spectrometry and 1H-nuclear magnetic resonance spectrometry of this metabolite, produced both by incubating methohexital with isolated rat liver microsomes and by isolating this metabolite from rat urine.
| Original language | English |
|---|---|
| Pages (from-to) | 403-12 |
| Number of pages | 10 |
| Journal | Journal of Chromatography A |
| Volume | 226 |
| Issue number | 2 |
| Publication status | Published - 11 Dec 1981 |
Keywords
- Animals
- Chromatography, Gas
- Gas Chromatography-Mass Spectrometry
- Humans
- Magnetic Resonance Spectroscopy
- Methohexital
- Microsomes, Liver
- Rats
- Comparative Study
- Journal Article
- Research Support, Non-U.S. Gov't
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