Single-Cell Acoustic Force Spectroscopy: Resolving Kinetics and Strength of T Cell Adhesion to Fibronectin

Douwe Kamsma, Pascal Bochet, Felix Oswald, Nander Alblas, Sophie Goyard, Gijs J.L. Wuite, Erwin J.G. Peterman, Thierry Rose

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Assessing the strength and kinetics of molecular interactions of cells with the extracellular matrix is fundamental to understand cell adhesion processes. Given the relevance of these processes, there is a strong need for physical methods to quantitatively assess the mechanism of cell adhesion at the single-cell level, allowing discrimination of cells with different behaviors. Here we introduce single-cell acoustic force spectroscopy (scAFS), an approach that makes use of acoustic waves to exert controlled forces, up to 1 nN, to hundreds of individual cells in parallel. We demonstrate the potential of scAFS by measuring adhesion forces and kinetics of CD4+ T lymphocytes (CD4) to fibronectin. We determined that CD4 adhesion is accelerated by interleukin-7, their main regulatory cytokine, whereas CD4 binding strength remains the same. Activation of these cells likely increases their chance to bind to the vessel wall in the blood flow to infiltrate inflamed tissues and locally coordinate the immune response. Kamsma et al. introduce single-cell acoustic force spectroscopy (scAFS), an approach using acoustic waves to exert controlled forces to hundreds of individual cells in parallel in order to measure their adhesion forces and kinetics to targets in living conditions, opening up a wide range of potential applications in research and the clinic.

Original languageEnglish
Pages (from-to)3008-3016
Number of pages9
JournalCell Reports
Volume24
Issue number11
DOIs
Publication statusPublished - 11 Sep 2018

Fingerprint

T-cells
Cell adhesion
Fibronectins
Acoustics
Cell Adhesion
Spectrum Analysis
Adhesion
Spectroscopy
T-Lymphocytes
Kinetics
Acoustic waves
Interleukin-7
Molecular interactions
Blood
Chemical activation
Tissue
Cytokines
Social Conditions
Cell Communication
Extracellular Matrix

Keywords

  • acoustic force spectroscopy
  • adhesion kinetics
  • adhesion strength
  • CD4
  • extracellular matrix
  • fibronectin
  • integrin
  • mechanobiology
  • rupture force
  • T lymphocytes

Cite this

Kamsma, Douwe ; Bochet, Pascal ; Oswald, Felix ; Alblas, Nander ; Goyard, Sophie ; Wuite, Gijs J.L. ; Peterman, Erwin J.G. ; Rose, Thierry. / Single-Cell Acoustic Force Spectroscopy : Resolving Kinetics and Strength of T Cell Adhesion to Fibronectin. In: Cell Reports. 2018 ; Vol. 24, No. 11. pp. 3008-3016.
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Single-Cell Acoustic Force Spectroscopy : Resolving Kinetics and Strength of T Cell Adhesion to Fibronectin. / Kamsma, Douwe; Bochet, Pascal; Oswald, Felix; Alblas, Nander; Goyard, Sophie; Wuite, Gijs J.L.; Peterman, Erwin J.G.; Rose, Thierry.

In: Cell Reports, Vol. 24, No. 11, 11.09.2018, p. 3008-3016.

Research output: Contribution to JournalArticleAcademicpeer-review

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