Abstract
Assessing the strength and kinetics of molecular interactions of cells with the extracellular matrix is fundamental to understand cell adhesion processes. Given the relevance of these processes, there is a strong need for physical methods to quantitatively assess the mechanism of cell adhesion at the single-cell level, allowing discrimination of cells with different behaviors. Here we introduce single-cell acoustic force spectroscopy (scAFS), an approach that makes use of acoustic waves to exert controlled forces, up to 1 nN, to hundreds of individual cells in parallel. We demonstrate the potential of scAFS by measuring adhesion forces and kinetics of CD4+ T lymphocytes (CD4) to fibronectin. We determined that CD4 adhesion is accelerated by interleukin-7, their main regulatory cytokine, whereas CD4 binding strength remains the same. Activation of these cells likely increases their chance to bind to the vessel wall in the blood flow to infiltrate inflamed tissues and locally coordinate the immune response. Kamsma et al. introduce single-cell acoustic force spectroscopy (scAFS), an approach using acoustic waves to exert controlled forces to hundreds of individual cells in parallel in order to measure their adhesion forces and kinetics to targets in living conditions, opening up a wide range of potential applications in research and the clinic.
| Original language | English |
|---|---|
| Pages (from-to) | 3008-3016 |
| Number of pages | 9 |
| Journal | Cell Reports |
| Volume | 24 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - 11 Sep 2018 |
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Keywords
- acoustic force spectroscopy
- adhesion kinetics
- adhesion strength
- CD4
- extracellular matrix
- fibronectin
- integrin
- mechanobiology
- rupture force
- T lymphocytes
Cite this
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Single-Cell Acoustic Force Spectroscopy : Resolving Kinetics and Strength of T Cell Adhesion to Fibronectin. / Kamsma, Douwe; Bochet, Pascal; Oswald, Felix; Alblas, Nander; Goyard, Sophie; Wuite, Gijs J.L.; Peterman, Erwin J.G.; Rose, Thierry.
In: Cell Reports, Vol. 24, No. 11, 11.09.2018, p. 3008-3016.Research output: Contribution to Journal › Article › Academic › peer-review
TY - JOUR
T1 - Single-Cell Acoustic Force Spectroscopy
T2 - Resolving Kinetics and Strength of T Cell Adhesion to Fibronectin
AU - Kamsma, Douwe
AU - Bochet, Pascal
AU - Oswald, Felix
AU - Alblas, Nander
AU - Goyard, Sophie
AU - Wuite, Gijs J.L.
AU - Peterman, Erwin J.G.
AU - Rose, Thierry
PY - 2018/9/11
Y1 - 2018/9/11
N2 - Assessing the strength and kinetics of molecular interactions of cells with the extracellular matrix is fundamental to understand cell adhesion processes. Given the relevance of these processes, there is a strong need for physical methods to quantitatively assess the mechanism of cell adhesion at the single-cell level, allowing discrimination of cells with different behaviors. Here we introduce single-cell acoustic force spectroscopy (scAFS), an approach that makes use of acoustic waves to exert controlled forces, up to 1 nN, to hundreds of individual cells in parallel. We demonstrate the potential of scAFS by measuring adhesion forces and kinetics of CD4+ T lymphocytes (CD4) to fibronectin. We determined that CD4 adhesion is accelerated by interleukin-7, their main regulatory cytokine, whereas CD4 binding strength remains the same. Activation of these cells likely increases their chance to bind to the vessel wall in the blood flow to infiltrate inflamed tissues and locally coordinate the immune response. Kamsma et al. introduce single-cell acoustic force spectroscopy (scAFS), an approach using acoustic waves to exert controlled forces to hundreds of individual cells in parallel in order to measure their adhesion forces and kinetics to targets in living conditions, opening up a wide range of potential applications in research and the clinic.
AB - Assessing the strength and kinetics of molecular interactions of cells with the extracellular matrix is fundamental to understand cell adhesion processes. Given the relevance of these processes, there is a strong need for physical methods to quantitatively assess the mechanism of cell adhesion at the single-cell level, allowing discrimination of cells with different behaviors. Here we introduce single-cell acoustic force spectroscopy (scAFS), an approach that makes use of acoustic waves to exert controlled forces, up to 1 nN, to hundreds of individual cells in parallel. We demonstrate the potential of scAFS by measuring adhesion forces and kinetics of CD4+ T lymphocytes (CD4) to fibronectin. We determined that CD4 adhesion is accelerated by interleukin-7, their main regulatory cytokine, whereas CD4 binding strength remains the same. Activation of these cells likely increases their chance to bind to the vessel wall in the blood flow to infiltrate inflamed tissues and locally coordinate the immune response. Kamsma et al. introduce single-cell acoustic force spectroscopy (scAFS), an approach using acoustic waves to exert controlled forces to hundreds of individual cells in parallel in order to measure their adhesion forces and kinetics to targets in living conditions, opening up a wide range of potential applications in research and the clinic.
KW - acoustic force spectroscopy
KW - adhesion kinetics
KW - adhesion strength
KW - CD4
KW - extracellular matrix
KW - fibronectin
KW - integrin
KW - mechanobiology
KW - rupture force
KW - T lymphocytes
UR - http://www.scopus.com/inward/record.url?scp=85052639603&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85052639603&partnerID=8YFLogxK
U2 - 10.1016/j.celrep.2018.08.034
DO - 10.1016/j.celrep.2018.08.034
M3 - Article
VL - 24
SP - 3008
EP - 3016
JO - Cell Reports
JF - Cell Reports
SN - 2211-1247
IS - 11
ER -