Single-Cell Measurements Using Acoustic Force Spectroscopy (AFS)

Kees Karel H. Taris; Douwe Kamsma; Gijs J.L. Wuite

doi:10.1007/978-1-0716-3377-9_22

Single-Cell Measurements Using Acoustic Force Spectroscopy (AFS)

Kees Karel H. Taris, Douwe Kamsma, Gijs J.L. Wuite^*

^*Corresponding author for this work

Research output: Chapter in Book / Report / Conference proceeding › Chapter › Academic › peer-review

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Abstract

Single-molecule force spectroscopy is a powerful tool to investigate the forces and motions related to interactions of biological molecules. Acoustic force spectroscopy (AFS) is a developed measurement tool to study single molecules or cells making use of acoustic standing waves. AFS permits high experimental throughput because many individual molecules can be manipulated and tracked in parallel. Moreover, a wide range of forces can be applied as well as a force loading rate with range of six orders of magnitude. At the same time, AFS stands out because of its simplicity and the compactness of the experimental setup. Even though the AFS setup is simple, it can still be challenging to perform high-quality measurements. Here we describe, in detail, how to setup, perform, and analyze an AFS measurement to determine cell adhesion.

Original language	English
Title of host publication	Single molecule analysis
Subtitle of host publication	Methods and protocols
Editors	Iddo Heller, David Dulin, Erwin J.G. Peterman
Publisher	Humana Press Inc
Pages	467-477
Number of pages	11
Edition	3rd
ISBN (Electronic)	9781071633779
ISBN (Print)	9781071633762, 9781071633793
DOIs	https://doi.org/10.1007/978-1-0716-3377-9_22
Publication status	Published - 2024

Publication series

Name	Methods in Molecular Biology
Volume	2694
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Funding Information:
We thank P. Bouti (Sanquin Amsterdam) for the cells and R. Sorkin for collaborating and discussing results. We acknowledge support by NWO (Natuurkunde—Projectruimte 2017-I, 680-91-102) (GJLW).

Publisher Copyright:
© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.

Funding

We thank P. Bouti (Sanquin Amsterdam) for the cells and R. Sorkin for collaborating and discussing results. We acknowledge support by NWO (Natuurkunde—Projectruimte 2017-I, 680-91-102) (GJLW).

Keywords

Acoustic force spectroscopy (AFS)
Cell adhesion
Molecular biophysics
Multiplexed single-molecule method

Access to Document

10.1007/978-1-0716-3377-9_22

Taris_Kamsma_Wuite_2024_978-1-0716-3376-2_chap22Submitted manuscript, 1.1 MB

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Single-Cell Measurements Using Acoustic Force Spectroscopy (AFS). / Taris, Kees Karel H.; Kamsma, Douwe; Wuite, Gijs J.L.
Single molecule analysis: Methods and protocols. ed. / Iddo Heller; David Dulin; Erwin J.G. Peterman. 3rd. ed. Humana Press Inc, 2024. p. 467-477 (Methods in Molecular Biology; Vol. 2694).

Research output: Chapter in Book / Report / Conference proceeding › Chapter › Academic › peer-review

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Single-Cell Measurements Using Acoustic Force Spectroscopy (AFS)

Abstract

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