TY - JOUR
T1 - Single metallic nanoparticle imaging for protein detection in cells
AU - Cognet, L.
AU - Tardin, C.
AU - Boyer, D.
AU - Choquet, D.
AU - Tamarat, P.
AU - Lounis, B.
N1 - Single metallic nanoparticle imaging for protein detection in cells
PY - 2003
Y1 - 2003
N2 - We performed a visualization of membrane proteins labeled with 10-nm gold nanoparticles in cells, using an all-optical method based on photothermal interference contrast. The high sensitivity of the method and the stability of the signals allows 3D imaging of individual nanoparticles without the drawbacks of photobleaching and blinking inherent to fluorescent markers. A simple analytical model is derived to account for the measurements of the signal amplitude and the spatial resolution. The photothermal interference contrast method provides an efficient, reproducible, and promising way to visualize low amounts of proteins in cells by optical means.
AB - We performed a visualization of membrane proteins labeled with 10-nm gold nanoparticles in cells, using an all-optical method based on photothermal interference contrast. The high sensitivity of the method and the stability of the signals allows 3D imaging of individual nanoparticles without the drawbacks of photobleaching and blinking inherent to fluorescent markers. A simple analytical model is derived to account for the measurements of the signal amplitude and the spatial resolution. The photothermal interference contrast method provides an efficient, reproducible, and promising way to visualize low amounts of proteins in cells by optical means.
UR - https://www.scopus.com/pages/publications/0141816809
UR - https://www.scopus.com/inward/citedby.url?scp=0141816809&partnerID=8YFLogxK
U2 - 10.1073/pnas.1534635100
DO - 10.1073/pnas.1534635100
M3 - Article
SN - 0027-8424
VL - 100
SP - 11350
EP - 11355
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 20
ER -