Single-molecule fluorescence microscopy in living Caenorhabditis elegans

Research output: Chapter in Book / Report / Conference proceedingChapterAcademic

Abstract

Transportation of organelles and biomolecules is vital for many cellular processes. Single-molecule (SM) fluorescence microscopy can expose molecular aspects of the dynamics that remain unresolved in ensemble experiments. For example, trajectories of individual, moving biomolecules can reveal velocity and changes therein, including pauses. We use SM imaging to study the dynamics of motor proteins and their cargo in the cilia of living C. elegans. To this end, we employ standard fluorescent proteins, an epi-illuminated, wide-field fluorescence microscope and mostly open-source software. This chapter describes the setup we use, the preparation of samples, a protocol for single-molecule imaging in C. elegans and data analysis.

Original languageEnglish
Title of host publicationSingle Molecule Analysis
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc
Pages145-154
Number of pages10
ISBN (Electronic)9781493972715
DOIs
Publication statusPublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1665
ISSN (Print)1064-3745

Fingerprint

Caenorhabditis elegans
Fluorescence Microscopy
Cilia
Molecular Dynamics Simulation
Organelles
Proteins
Software
Fluorescence
Single Molecule Imaging

Keywords

  • Caenorhabditis elegans
  • Live-cell imaging
  • Single-molecule imaging
  • Wide-field fluorescence microscopy

Cite this

van Krugten, J., & Peterman, E. J. G. (2018). Single-molecule fluorescence microscopy in living Caenorhabditis elegans. In Single Molecule Analysis: Methods and Protocols (pp. 145-154). (Methods in Molecular Biology; Vol. 1665). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7271-5_8
van Krugten, Jaap ; Peterman, Erwin J.G. / Single-molecule fluorescence microscopy in living Caenorhabditis elegans. Single Molecule Analysis: Methods and Protocols. Humana Press Inc, 2018. pp. 145-154 (Methods in Molecular Biology).
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van Krugten, J & Peterman, EJG 2018, Single-molecule fluorescence microscopy in living Caenorhabditis elegans. in Single Molecule Analysis: Methods and Protocols. Methods in Molecular Biology, vol. 1665, Humana Press Inc, pp. 145-154. https://doi.org/10.1007/978-1-4939-7271-5_8

Single-molecule fluorescence microscopy in living Caenorhabditis elegans. / van Krugten, Jaap; Peterman, Erwin J.G.

Single Molecule Analysis: Methods and Protocols. Humana Press Inc, 2018. p. 145-154 (Methods in Molecular Biology; Vol. 1665).

Research output: Chapter in Book / Report / Conference proceedingChapterAcademic

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van Krugten J, Peterman EJG. Single-molecule fluorescence microscopy in living Caenorhabditis elegans. In Single Molecule Analysis: Methods and Protocols. Humana Press Inc. 2018. p. 145-154. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7271-5_8