Single-molecule imaging of Escherichia coli transmembrane proteins

A. Varadajan, F. Oswald, Y.J.M. Bollen

Research output: Chapter in Book / Report / Conference proceedingChapterAcademicpeer-review

Abstract

Single-molecule imaging in living cells can provide unique information about biological processes. Bacteria offer some particular challenges for single-molecule imaging due to their small size, only slightly larger than the diffraction limit of visible light. Here, we describe how reliable and reproducible single-molecule data can be obtained for a transmembrane protein in the Gram-negative bacterium Escherichia coli by using live-cell fluorescence microscopy. Fluorescent labeling of a protein by genetic fusion, cell culturing, sample preparation, imaging, and data analysis are discussed.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
EditorsErwin Peterman
PublisherHumana Press Inc
Pages135-144
Number of pages10
Volume1665
ISBN (Electronic)9781493972715
ISBN (Print)9781493972708
DOIs
Publication statusPublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1665
ISSN (Print)1064-3745

Fingerprint

Escherichia coli Proteins
Biological Phenomena
Cell Fusion
Gram-Negative Bacteria
Fluorescence Microscopy
Proteins
Escherichia coli
Bacteria
Light
Single Molecule Imaging

Keywords

  • Bacteria
  • Diffusion
  • Escherichia coli
  • Fluorescence microscopy
  • Sample preparation
  • Single-molecule tracking
  • Transmembrane protein

Cite this

Varadajan, A., Oswald, F., & Bollen, Y. J. M. (2018). Single-molecule imaging of Escherichia coli transmembrane proteins. In E. Peterman (Ed.), Methods in Molecular Biology (Vol. 1665, pp. 135-144). (Methods in Molecular Biology; Vol. 1665). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7271-5_7
Varadajan, A. ; Oswald, F. ; Bollen, Y.J.M. / Single-molecule imaging of Escherichia coli transmembrane proteins. Methods in Molecular Biology. editor / Erwin Peterman. Vol. 1665 Humana Press Inc, 2018. pp. 135-144 (Methods in Molecular Biology).
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Varadajan, A, Oswald, F & Bollen, YJM 2018, Single-molecule imaging of Escherichia coli transmembrane proteins. in E Peterman (ed.), Methods in Molecular Biology. vol. 1665, Methods in Molecular Biology, vol. 1665, Humana Press Inc, pp. 135-144. https://doi.org/10.1007/978-1-4939-7271-5_7

Single-molecule imaging of Escherichia coli transmembrane proteins. / Varadajan, A.; Oswald, F.; Bollen, Y.J.M.

Methods in Molecular Biology. ed. / Erwin Peterman. Vol. 1665 Humana Press Inc, 2018. p. 135-144 (Methods in Molecular Biology; Vol. 1665).

Research output: Chapter in Book / Report / Conference proceedingChapterAcademicpeer-review

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KW - Bacteria

KW - Diffusion

KW - Escherichia coli

KW - Fluorescence microscopy

KW - Sample preparation

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Varadajan A, Oswald F, Bollen YJM. Single-molecule imaging of Escherichia coli transmembrane proteins. In Peterman E, editor, Methods in Molecular Biology. Vol. 1665. Humana Press Inc. 2018. p. 135-144. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7271-5_7