Single-molecule imaging of Escherichia coli transmembrane proteins

A. Varadajan, F. Oswald, Y.J.M. Bollen*

*Corresponding author for this work

Research output: Chapter in Book / Report / Conference proceedingChapterAcademicpeer-review


Single-molecule imaging in living cells can provide unique information about biological processes. Bacteria offer some particular challenges for single-molecule imaging due to their small size, only slightly larger than the diffraction limit of visible light. Here, we describe how reliable and reproducible single-molecule data can be obtained for a transmembrane protein in the Gram-negative bacterium Escherichia coli by using live-cell fluorescence microscopy. Fluorescent labeling of a protein by genetic fusion, cell culturing, sample preparation, imaging, and data analysis are discussed.

Original languageEnglish
Title of host publicationSingle Molecule Analysis
Subtitle of host publicationMethods and Protocols
EditorsErwin Peterman
PublisherHumana Press Inc
Number of pages10
ISBN (Electronic)9781493972715
ISBN (Print)9781493972708
Publication statusPublished - 2018

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Bacteria
  • Diffusion
  • Escherichia coli
  • Fluorescence microscopy
  • Sample preparation
  • Single-molecule tracking
  • Transmembrane protein


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