@inbook{60bce6689b7b433c982645c1c7f83982,
title = "Single-molecule imaging of Escherichia coli transmembrane proteins",
abstract = "Single-molecule imaging in living cells can provide unique information about biological processes. Bacteria offer some particular challenges for single-molecule imaging due to their small size, only slightly larger than the diffraction limit of visible light. Here, we describe how reliable and reproducible single-molecule data can be obtained for a transmembrane protein in the Gram-negative bacterium Escherichia coli by using live-cell fluorescence microscopy. Fluorescent labeling of a protein by genetic fusion, cell culturing, sample preparation, imaging, and data analysis are discussed.",
keywords = "Bacteria, Diffusion, Escherichia coli, Fluorescence microscopy, Sample preparation, Single-molecule tracking, Transmembrane protein",
author = "A. Varadajan and F. Oswald and Y.J.M. Bollen",
year = "2018",
doi = "10.1007/978-1-4939-7271-5_7",
language = "English",
isbn = "9781493972708",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc",
pages = "135--144",
editor = "Erwin Peterman",
booktitle = "Single Molecule Analysis",
}