Single-molecule measurements using acoustic force spectroscopy (AFS)

Douwe Kamsma, Gijs J.L. Wuite

Research output: Chapter in Book / Report / Conference proceedingChapterAcademicpeer-review

Abstract

Single-molecule force spectroscopy is a powerful tool to investigate the forces and motions related to interactions of biological molecules. Acoustic Force Spectroscopy (AFS) is a recently developed measurement tool to study single molecules making use of acoustic standing waves. AFS permits high experimental throughput, because many individual molecules can be manipulated and tracked in parallel. Moreover, a wide range of forces can be applied, as well as a force loading rate with range of six orders of magnitude. At the same time, AFS stands out because of its simplicity and the compactness of the experimental setup. Even though the AFS setup is simple, it can still be challenging to perform high-quality measurements. Here we describe, in detail, how to setup, perform, and analyze an AFS measurement.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Place of PublicationNew York, NY
PublisherHumana Press Inc
Pages341-351
Number of pages11
DOIs
Publication statusPublished - Jan 2018

Publication series

NameMethods in Molecular Biology
Volume1665
ISSN (Print)1064-3745

Fingerprint

Acoustics
Spectrum Analysis

Keywords

  • Acoustic Force Spectroscopy (AFS)
  • DNA tethering
  • Molecular biophysics
  • Multiplexed single-molecule method

Cite this

Kamsma, D., & Wuite, G. J. L. (2018). Single-molecule measurements using acoustic force spectroscopy (AFS). In Methods in Molecular Biology (pp. 341-351). (Methods in Molecular Biology; Vol. 1665). New York, NY: Humana Press Inc. https://doi.org/10.1007/978-1-4939-7271-5_18
Kamsma, Douwe ; Wuite, Gijs J.L. / Single-molecule measurements using acoustic force spectroscopy (AFS). Methods in Molecular Biology. New York, NY : Humana Press Inc, 2018. pp. 341-351 (Methods in Molecular Biology).
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Kamsma, D & Wuite, GJL 2018, Single-molecule measurements using acoustic force spectroscopy (AFS). in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1665, Humana Press Inc, New York, NY, pp. 341-351. https://doi.org/10.1007/978-1-4939-7271-5_18

Single-molecule measurements using acoustic force spectroscopy (AFS). / Kamsma, Douwe; Wuite, Gijs J.L.

Methods in Molecular Biology. New York, NY : Humana Press Inc, 2018. p. 341-351 (Methods in Molecular Biology; Vol. 1665).

Research output: Chapter in Book / Report / Conference proceedingChapterAcademicpeer-review

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Kamsma D, Wuite GJL. Single-molecule measurements using acoustic force spectroscopy (AFS). In Methods in Molecular Biology. New York, NY: Humana Press Inc. 2018. p. 341-351. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7271-5_18