Single-molecule measurements using acoustic force spectroscopy (AFS)

Douwe Kamsma; Gijs J.L. Wuite

doi:10.1007/978-1-4939-7271-5_18

Single-molecule measurements using acoustic force spectroscopy (AFS)

Douwe Kamsma, Gijs J.L. Wuite^*

^*Corresponding author for this work

Research output: Chapter in Book / Report / Conference proceeding › Chapter › Academic › peer-review

Abstract

Single-molecule force spectroscopy is a powerful tool to investigate the forces and motions related to interactions of biological molecules. Acoustic Force Spectroscopy (AFS) is a recently developed measurement tool to study single molecules making use of acoustic standing waves. AFS permits high experimental throughput, because many individual molecules can be manipulated and tracked in parallel. Moreover, a wide range of forces can be applied, as well as a force loading rate with range of six orders of magnitude. At the same time, AFS stands out because of its simplicity and the compactness of the experimental setup. Even though the AFS setup is simple, it can still be challenging to perform high-quality measurements. Here we describe, in detail, how to setup, perform, and analyze an AFS measurement.

Original language	English
Title of host publication	Single Molecule Analysis
Subtitle of host publication	Methods and Protocols
Editors	Erwin J.G. Peterman
Place of Publication	New York, NY
Publisher	Humana Press Inc
Pages	341-351
Number of pages	11
Edition	2
ISBN (Electronic)	9781493972715
ISBN (Print)	9781493972708, 9781493984374
DOIs	https://doi.org/10.1007/978-1-4939-7271-5_18
Publication status	Published - Jan 2018

Publication series

Name	Methods in Molecular Biology
Volume	1665
ISSN (Print)	1064-3745

Funding

We thank A.S. Biebricher, S.E.D. Haene, and S. Acar for biochemical help; F. Oswald and G. Sitters for collaborating and discussing results. We acknowledge support by NWO VICI as well as a European Research Council (ERC) starting grant (G.J.L.W.).

Funders	Funder number
European Research Council
Nederlandse Organisatie voor Wetenschappelijk Onderzoek

Keywords

Acoustic Force Spectroscopy (AFS)
DNA tethering
Molecular biophysics
Multiplexed single-molecule method

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10.1007/978-1-4939-7271-5_18

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abstract = "Single-molecule force spectroscopy is a powerful tool to investigate the forces and motions related to interactions of biological molecules. Acoustic Force Spectroscopy (AFS) is a recently developed measurement tool to study single molecules making use of acoustic standing waves. AFS permits high experimental throughput, because many individual molecules can be manipulated and tracked in parallel. Moreover, a wide range of forces can be applied, as well as a force loading rate with range of six orders of magnitude. At the same time, AFS stands out because of its simplicity and the compactness of the experimental setup. Even though the AFS setup is simple, it can still be challenging to perform high-quality measurements. Here we describe, in detail, how to setup, perform, and analyze an AFS measurement.",

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Single-molecule measurements using acoustic force spectroscopy (AFS). / Kamsma, Douwe; Wuite, Gijs J.L.
Single Molecule Analysis: Methods and Protocols. ed. / Erwin J.G. Peterman. 2. ed. New York, NY: Humana Press Inc, 2018. p. 341-351 (Methods in Molecular Biology; Vol. 1665).

Research output: Chapter in Book / Report / Conference proceeding › Chapter › Academic › peer-review

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Single-molecule measurements using acoustic force spectroscopy (AFS)

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