Single-Molecule Turnarounds of Intraflagellar Transport at the C. elegans Ciliary Tip

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Cilia are microtubule-based sensing hubs that rely on intraflagellar transport (IFT) for their development, maintenance, and function. Kinesin-2 motors transport IFT trains, consisting of IFT proteins and cargo, from ciliary base to tip. There, trains turn around and are transported back by IFT dynein. The mechanism of tip turnaround has remained elusive. Here, we employ single-molecule fluorescence microscopy of IFT components in the tips of phasmid cilia of living C. elegans. Analysis of the trajectories reveals that while motor proteins and IFT-A particle component CHE-11 mostly turn around immediately, the IFT-B particle component OSM-6 pauses for several seconds. Our data indicate that IFT trains disassemble into at least IFT-A, IFT-B, IFT-dynein, and OSM-3 complexes at the tip, where OSM-6 is temporarily retained or undergoes modification, prior to train reassembly and retrograde transport. The single-molecule approach used here is a valuable tool to study how directional switches occur in microtubule-based transport processes. Using single-molecule fluorescence microscopy, Mijalkovic et al. visualize the dynamics of IFT components at the tips of C. elegans chemosensory cilia. They find that the motors and the IFT-A particle component CHE-11 reverse almost immediately, while the IFT-B component OSM-6 is temporarily retained before reassembly and reversal.

Original languageEnglish
Pages (from-to)1701-1707.e2
Number of pages10
JournalCell Reports
Volume25
Issue number7
DOIs
Publication statusPublished - 13 Nov 2018

Fingerprint

Cilia
Dyneins
Fluorescence microscopy
Fluorescence Microscopy
Microtubules
Molecules
Kinesin
Protein Transport
Carrier Proteins
Switches
Trajectories
Maintenance
Proteins
Single Molecule Imaging

Keywords

  • ciliary tip turns
  • dynein
  • IFT
  • intracellular transport
  • kinesin
  • single-molecule biophysics

Cite this

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title = "Single-Molecule Turnarounds of Intraflagellar Transport at the C. elegans Ciliary Tip",
abstract = "Cilia are microtubule-based sensing hubs that rely on intraflagellar transport (IFT) for their development, maintenance, and function. Kinesin-2 motors transport IFT trains, consisting of IFT proteins and cargo, from ciliary base to tip. There, trains turn around and are transported back by IFT dynein. The mechanism of tip turnaround has remained elusive. Here, we employ single-molecule fluorescence microscopy of IFT components in the tips of phasmid cilia of living C. elegans. Analysis of the trajectories reveals that while motor proteins and IFT-A particle component CHE-11 mostly turn around immediately, the IFT-B particle component OSM-6 pauses for several seconds. Our data indicate that IFT trains disassemble into at least IFT-A, IFT-B, IFT-dynein, and OSM-3 complexes at the tip, where OSM-6 is temporarily retained or undergoes modification, prior to train reassembly and retrograde transport. The single-molecule approach used here is a valuable tool to study how directional switches occur in microtubule-based transport processes. Using single-molecule fluorescence microscopy, Mijalkovic et al. visualize the dynamics of IFT components at the tips of C. elegans chemosensory cilia. They find that the motors and the IFT-A particle component CHE-11 reverse almost immediately, while the IFT-B component OSM-6 is temporarily retained before reassembly and reversal.",
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Single-Molecule Turnarounds of Intraflagellar Transport at the C. elegans Ciliary Tip. / Mijalkovic, Jona; van Krugten, Jaap; Oswald, Felix; Acar, Seyda; Peterman, Erwin J.G.

In: Cell Reports, Vol. 25, No. 7, 13.11.2018, p. 1701-1707.e2.

Research output: Contribution to JournalArticleAcademicpeer-review

TY - JOUR

T1 - Single-Molecule Turnarounds of Intraflagellar Transport at the C. elegans Ciliary Tip

AU - Mijalkovic, Jona

AU - van Krugten, Jaap

AU - Oswald, Felix

AU - Acar, Seyda

AU - Peterman, Erwin J.G.

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AB - Cilia are microtubule-based sensing hubs that rely on intraflagellar transport (IFT) for their development, maintenance, and function. Kinesin-2 motors transport IFT trains, consisting of IFT proteins and cargo, from ciliary base to tip. There, trains turn around and are transported back by IFT dynein. The mechanism of tip turnaround has remained elusive. Here, we employ single-molecule fluorescence microscopy of IFT components in the tips of phasmid cilia of living C. elegans. Analysis of the trajectories reveals that while motor proteins and IFT-A particle component CHE-11 mostly turn around immediately, the IFT-B particle component OSM-6 pauses for several seconds. Our data indicate that IFT trains disassemble into at least IFT-A, IFT-B, IFT-dynein, and OSM-3 complexes at the tip, where OSM-6 is temporarily retained or undergoes modification, prior to train reassembly and retrograde transport. The single-molecule approach used here is a valuable tool to study how directional switches occur in microtubule-based transport processes. Using single-molecule fluorescence microscopy, Mijalkovic et al. visualize the dynamics of IFT components at the tips of C. elegans chemosensory cilia. They find that the motors and the IFT-A particle component CHE-11 reverse almost immediately, while the IFT-B component OSM-6 is temporarily retained before reassembly and reversal.

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