Abstract
G protein coupled receptors (GPCRs) represent a major superfamily of transmembrane receptor proteins that are crucial in cellular signaling and are major pharmacological targets. While the activity of GPCRs can be modulated by agonist binding, the mechanisms that link agonist binding to G protein coupling are poorly understood. Here we present a method to accurately examine the activity of ligands in their bound state, even at low affinity, by solid-state NMR dipolar correlation spectroscopy and confront this method with the human H
Original language | English |
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Pages (from-to) | 867-72 |
Journal | Journal of the American Chemical Society |
Volume | 129 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2007 |