Sortase A as a tool to functionalize surfaces

T. Sijbrandij, N. Cukkemane, K. Nazmi, E.C.I. Veerman, F.J. Bikker

Research output: Contribution to JournalArticleAcademicpeer-review


A widely accepted approach to combat surface fouling is based on the prevention of biofoulants to attach to a surface by the functionalization with poly(ethylene glycol) (PEG). The goal of this study was to generate a proof of concept for the enzymatic coupling of PEG to a peptide precoated surface by using the enzyme Sortase A (SrtA). A hydrophobic polystyrene surface was primed with anchoring peptide P3 equipped with a pentaglycine acceptor motif for SrtA, to enable subsequent transpeptidation with either biotin or a PEG-tail containing the sortase recognition motif LPETG. High levels of surface-bound biotin were detected only in cases with biotin-LPETG and SrtA. Little if any reactivity was detected in wells treated with the SrtA scrambled motif EGLTP, or in the absence of SrtA. Conjugation of PEG resulted in a significant decrease of bacterial adherence to the surface.
Original languageEnglish
Pages (from-to)828-831
JournalBioconjugate chemistry
Issue number5
Publication statusPublished - 2013


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