TY - JOUR
T1 - Specificity of circulating antigen detection for schistosomiasis mansoni in Senegal and Burundi
AU - Polman, Katja
AU - Diakhate, Meremarie M.
AU - Engels, Dirk
AU - Nahimana, Samuel
AU - Van Dam, Govert J.
AU - Falcão Ferreira, Sonja T M
AU - Deelder, Andre M.
AU - Gryseels, Bruno
PY - 2000
Y1 - 2000
N2 - The specificity of schistosome circulating antigen detection was determined in negative individuals from two S. mansoni- endemic countries, Senegal and Burundi, and compared with results from Dutch control individuals. A nearly absolute specificity was achieved for circulating anodic antigen (CAA) detection in serum, irrespective of the target population or sample pretreatment method. Circulating cathodic antigen (CCA) detection in serum and urine resulted in a lower specificity than serum CAA detection. Apparent large differences in specificity of CCA detection between countries were mainly due to pretreatment methods. Apparently, the alkaline/heating pretreatment method is not as effective as trichloroacetic acid (TCA)-pretreatment in removing (certain) interfering components, which may vary between populations. In view of the development of the urine CCA assay into a noninvasive screening test, a slightly lower specificity may still be acceptable. For precise epidemiological analyses the highly specific serum CAA assay remains the method of choice.
AB - The specificity of schistosome circulating antigen detection was determined in negative individuals from two S. mansoni- endemic countries, Senegal and Burundi, and compared with results from Dutch control individuals. A nearly absolute specificity was achieved for circulating anodic antigen (CAA) detection in serum, irrespective of the target population or sample pretreatment method. Circulating cathodic antigen (CCA) detection in serum and urine resulted in a lower specificity than serum CAA detection. Apparent large differences in specificity of CCA detection between countries were mainly due to pretreatment methods. Apparently, the alkaline/heating pretreatment method is not as effective as trichloroacetic acid (TCA)-pretreatment in removing (certain) interfering components, which may vary between populations. In view of the development of the urine CCA assay into a noninvasive screening test, a slightly lower specificity may still be acceptable. For precise epidemiological analyses the highly specific serum CAA assay remains the method of choice.
KW - Circulating anodic antigen (CAA)
KW - Circulating cathodic antigen (CCA)
KW - Endemic countries
KW - Pretreatment
KW - Schistosoma mansoni
KW - Specificity
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U2 - 10.1046/j.1365-3156.2000.00600.x
DO - 10.1046/j.1365-3156.2000.00600.x
M3 - Article
C2 - 10995094
AN - SCOPUS:0033840938
SN - 1360-2276
VL - 5
SP - 534
EP - 537
JO - Tropical Medicine and International Health
JF - Tropical Medicine and International Health
IS - 8
ER -