Spectrally decomposed dark-to-light transitions in a PSI-deficient mutant of Synechocystis sp. PCC 6803

Alonso M. Acuña; Pascal van Alphen; Filipe Branco dos Santos; Rienk van Grondelle; Klaas J. Hellingwerf; Ivo H.M. van Stokkum

doi:10.1016/j.bbabio.2017.11.002

Spectrally decomposed dark-to-light transitions in a PSI-deficient mutant of Synechocystis sp. PCC 6803

Alonso M. Acuña, Pascal van Alphen, Filipe Branco dos Santos, Rienk van Grondelle, Klaas J. Hellingwerf, Ivo H.M. van Stokkum^*

^*Corresponding author for this work

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Abstract

Cyanobacterial thylakoid membranes are known to host photosynthetic and respiratory complexes. This hampers a straight forward interpretation of the highly dynamic fluorescence originating from photosynthetic units. The present study focuses on dark-to-light transitions in whole cells of a PSI-deficient mutant of the cyanobacterium Synechocystis sp. PCC 6803. The time-dependent cellular fluorescence spectrum has been measured, while having previously exposed the cells to different conditions that affect respiratory activity. The analysis method used allows the detected signal to be decomposed in a few components that are then assigned to functional emitting species. Additionally, we have worked out a minimal mathematical model consisting of sensible postulated species to interpret the recorded data. We conclude that the following two functional complexes play a major role: a phycobilisome antenna complex coupled to a PSII dimer with either two or no closed reaction centers. Crucially, we present evidence for an additional species capable of strongly quenching fluorescence, whose formation requires the presence of oxygen.

Original language	English
Pages (from-to)	57-68
Number of pages	12
Journal	Biochimica et Biophysica Acta - Bioenergetics
Volume	1859
Issue number	2
Early online date	11 Nov 2017
DOIs	https://doi.org/10.1016/j.bbabio.2017.11.002
Publication status	Published - Feb 2018

Funding

Joris Snellenburg is thanked for helpful discussions. AMA gratefully acknowledges Michael Reus for his valuable assistance in re-building the multiple LED set-up in Amsterdam. Roberta Croce and Alfred Holzwarth are thanked for letting us use this set-up. The ΔPSI mutant which was originally constructed by Wim Vermaas (Arizona State University, Tempe, Arizona, U.S.) was kindly provided to us by Prof. Christiane Funk (Umeå University, Sweden). This research was performed as part of the BioSolar Cells research programme, sponsored by the Dutch Ministry of Economic Affairs . This work is part of the research program of the Netherlands Organization for Scientific Research (NWO, previously FOM). Appendix A

Funders	Funder number
Ministerie van Economische Zaken
Nederlandse Organisatie voor Wetenschappelijk Onderzoek

Keywords

Cyanobacteria
Singular Value Decomposition
Spectrally-resolved fluorometry
Time-resolved spectroscopy

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10.1016/j.bbabio.2017.11.002

Spectrally decomposed dark-to-light transitions in a PSI-deficient mutant of Synechocystis sp. PCC 6803Final published version, 1.62 MBLicence: Article 25fa Dutch Copyright Act

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title = "Spectrally decomposed dark-to-light transitions in a PSI-deficient mutant of Synechocystis sp. PCC 6803",

abstract = "Cyanobacterial thylakoid membranes are known to host photosynthetic and respiratory complexes. This hampers a straight forward interpretation of the highly dynamic fluorescence originating from photosynthetic units. The present study focuses on dark-to-light transitions in whole cells of a PSI-deficient mutant of the cyanobacterium Synechocystis sp. PCC 6803. The time-dependent cellular fluorescence spectrum has been measured, while having previously exposed the cells to different conditions that affect respiratory activity. The analysis method used allows the detected signal to be decomposed in a few components that are then assigned to functional emitting species. Additionally, we have worked out a minimal mathematical model consisting of sensible postulated species to interpret the recorded data. We conclude that the following two functional complexes play a major role: a phycobilisome antenna complex coupled to a PSII dimer with either two or no closed reaction centers. Crucially, we present evidence for an additional species capable of strongly quenching fluorescence, whose formation requires the presence of oxygen.",

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AU - Acuña, Alonso M.

AU - van Alphen, Pascal

AU - Branco dos Santos, Filipe

AU - van Grondelle, Rienk

AU - Hellingwerf, Klaas J.

AU - van Stokkum, Ivo H.M.

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AB - Cyanobacterial thylakoid membranes are known to host photosynthetic and respiratory complexes. This hampers a straight forward interpretation of the highly dynamic fluorescence originating from photosynthetic units. The present study focuses on dark-to-light transitions in whole cells of a PSI-deficient mutant of the cyanobacterium Synechocystis sp. PCC 6803. The time-dependent cellular fluorescence spectrum has been measured, while having previously exposed the cells to different conditions that affect respiratory activity. The analysis method used allows the detected signal to be decomposed in a few components that are then assigned to functional emitting species. Additionally, we have worked out a minimal mathematical model consisting of sensible postulated species to interpret the recorded data. We conclude that the following two functional complexes play a major role: a phycobilisome antenna complex coupled to a PSII dimer with either two or no closed reaction centers. Crucially, we present evidence for an additional species capable of strongly quenching fluorescence, whose formation requires the presence of oxygen.

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Spectrally decomposed dark-to-light transitions in a PSI-deficient mutant of Synechocystis sp. PCC 6803

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