Abstract
The enzyme NADPH:protochlorophyllide oxidoreductase (POR) catalyses the reduction of protochlorophyllide into chlorophyllide, a precursor of chlorophyll a in photosynthetic organisms. The enzyme binds the substrate and the cofactor in the dark and catalysis is initiated by the absorption of light by the substrate. We have carried out spectroscopic measurements with ultrafast time resolution under single pulse conditions, which reveal a biphasic formation of the first catalytic intermediate, I675* with average rates of (3.7 ± 0.7 ps)
Original language | English |
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Pages (from-to) | 616-625 |
Journal | Physical Chemistry Chemical Physics - PCCP |
Volume | 14 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2012 |