Spectroscopic characterization of the first ultrafast catalytic intermediate in protochlorophyllide oxidoreductase

O. Sytina, I.H.M. van Stokkum, D.J. Heyes, C.N. Hunter, M.L. Groot

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The enzyme NADPH:protochlorophyllide oxidoreductase (POR) catalyses the reduction of protochlorophyllide into chlorophyllide, a precursor of chlorophyll a in photosynthetic organisms. The enzyme binds the substrate and the cofactor in the dark and catalysis is initiated by the absorption of light by the substrate. We have carried out spectroscopic measurements with ultrafast time resolution under single pulse conditions, which reveal a biphasic formation of the first catalytic intermediate, I675* with average rates of (3.7 ± 0.7 ps)
Original languageEnglish
Pages (from-to)616-625
JournalPhysical Chemistry Chemical Physics - PCCP
Issue number2
Publication statusPublished - 2012


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