Stark fluorescence spectroscopy on peridinin–chlorophyll–protein complex of dinoflagellate, Amphidinium carterae

Anjue Mane Ara, Md Shakil Bin Kashem, Rienk van Grondelle, M.D. Wahadoszamen*

*Corresponding author for this work

Research output: Contribution to JournalArticle

Abstract

Because of their peculiar but intriguing photophysical properties, peridinin–chlorophyll–protein complexes (PCPs), the peripheral light-harvesting antenna complexes of photosynthetic dinoflagellates have been unique targets of multidimensional theoretical and experimental investigations over the last few decades. The major light-harvesting chlorophyll a (Chl a) pigments of PCP are hypothesized to be spectroscopically heterogeneous. To study the spectral heterogeneity in terms of electrostatic parameters, we, in this study, implemented Stark fluorescence spectroscopy on PCP isolated from the dinoflagellate Amphidinium carterae. The comprehensive theoretical modeling of the Stark fluorescence spectrum with the help of the conventional Liptay formalism revealed the simultaneous presence of three emission bands in the fluorescence spectrum of PCP recorded upon excitation of peridinin. The three emission bands are found to possess different sets of electrostatic parameters with essentially increasing magnitude of charge-transfer character from the blue to redder ones. The different magnitudes of electrostatic parameters give good support to the earlier proposition that the spectral heterogeneity in PCP results from emissive Chl a clusters anchored at a different sites and domains within the protein network.

Original languageEnglish
Pages (from-to)233–239
Number of pages7
JournalPhotosynthesis Research
Volume143
Issue number3
Early online date25 Nov 2019
DOIs
Publication statusPublished - Mar 2020

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Keywords

  • Charge-transfer states
  • Energy transfer
  • Light harvesting
  • Peridinin–chlorophyll–protein complexes
  • Spectral heterogeneity
  • Stark spectroscopy

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