Streptococcus mutans transcriptome in the presence of sodium fluoride and sucrose

N. Damé-Teixeira; D. Deng; T. Do

doi:10.1016/j.archoralbio.2019.04.020

Streptococcus mutans transcriptome in the presence of sodium fluoride and sucrose

N. Damé-Teixeira
, D. Deng
, T. Do

Preventive Dentistry

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Objective: Considering the diverse mechanisms by which fluoride could affect oral bacteria, this study evaluated the effect of sodium fluoride onStreptococcus mutans transcriptome in the presence of sucrose.
Methods: S. mutans UA159 was cultured in 3 different types of media: medium control[TY], sucrose control[TY_S], and sodium fluoride sucrose test[TY_S_NaF]. Triplicates of each group were sampled at exponential phase 3 h after inoculation, total RNA was isolated, mRNA enriched and cDNA paired-end sequenced (Illumina Hi-Seq2500).
Results: Genes related toS. mutans adhesion(gtfB and gtfC), acidogenicity and sugar transport were up-regulated in the presence of sucrose(TY_S) and sucrose/fluoride(TY_S_NaF), whereas gene dltA, D-alanine-activating enzyme, which is related to regulation of non-PTS sugar internalization was down-regulated. Up-regulation of the scrA gene and the PTS fructose-and mannose system, as well as functions such as those involved in stress and defence responses and peptidases; and down-regulation of lacACDG and pyruvate formate-lyase were observed in the TY_S_NaF group, as compared to TY_S group.
Conclusions: The presence of NaF has decreased the overall gene expression level inS. mutans. However, its major effect seems to be the inducing of expression of genes involved in some PEP:PTS systems and other metabolic transporters which imply specific cellular internalisation of sugars.

Original language	English
Pages (from-to)	186-192
Journal	Archives of Oral Biology
Volume	102
DOIs	https://doi.org/10.1016/j.archoralbio.2019.04.020
Publication status	Published - Jun 2019

Bibliographical note

Export Date: 22 October 2019

CODEN: AOBIA

Funding

This is an original research, not under publication consideration elsewhere, and was partly funded by the Leeds Hospital Charitable Foundation ( R&D/PP/12011 ).

Funders	Funder number
Leeds Hospital Charitable Foundation	R&D/PP/12011

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10.1016/j.archoralbio.2019.04.020

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title = "Streptococcus mutans transcriptome in the presence of sodium fluoride and sucrose",

abstract = "Objective: Considering the diverse mechanisms by which fluoride could affect oral bacteria, this study evaluated the effect of sodium fluoride onStreptococcus mutans transcriptome in the presence of sucrose. Methods: S. mutans UA159 was cultured in 3 different types of media: medium control[TY], sucrose control[TY\_S], and sodium fluoride sucrose test[TY\_S\_NaF]. Triplicates of each group were sampled at exponential phase 3 h after inoculation, total RNA was isolated, mRNA enriched and cDNA paired-end sequenced (Illumina Hi-Seq2500). Results: Genes related toS. mutans adhesion(gtfB and gtfC), acidogenicity and sugar transport were up-regulated in the presence of sucrose(TY\_S) and sucrose/fluoride(TY\_S\_NaF), whereas gene dltA, D-alanine-activating enzyme, which is related to regulation of non-PTS sugar internalization was down-regulated. Up-regulation of the scrA gene and the PTS fructose-and mannose system, as well as functions such as those involved in stress and defence responses and peptidases; and down-regulation of lacACDG and pyruvate formate-lyase were observed in the TY\_S\_NaF group, as compared to TY\_S group. Conclusions: The presence of NaF has decreased the overall gene expression level inS. mutans. However, its major effect seems to be the inducing of expression of genes involved in some PEP:PTS systems and other metabolic transporters which imply specific cellular internalisation of sugars.",

author = "N. Dam{\'e}-Teixeira and D. Deng and T. Do",

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year = "2019",

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doi = "10.1016/j.archoralbio.2019.04.020",

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AU - Damé-Teixeira, N.

AU - Deng, D.

AU - Do, T.

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PY - 2019/6

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N2 - Objective: Considering the diverse mechanisms by which fluoride could affect oral bacteria, this study evaluated the effect of sodium fluoride onStreptococcus mutans transcriptome in the presence of sucrose. Methods: S. mutans UA159 was cultured in 3 different types of media: medium control[TY], sucrose control[TY_S], and sodium fluoride sucrose test[TY_S_NaF]. Triplicates of each group were sampled at exponential phase 3 h after inoculation, total RNA was isolated, mRNA enriched and cDNA paired-end sequenced (Illumina Hi-Seq2500). Results: Genes related toS. mutans adhesion(gtfB and gtfC), acidogenicity and sugar transport were up-regulated in the presence of sucrose(TY_S) and sucrose/fluoride(TY_S_NaF), whereas gene dltA, D-alanine-activating enzyme, which is related to regulation of non-PTS sugar internalization was down-regulated. Up-regulation of the scrA gene and the PTS fructose-and mannose system, as well as functions such as those involved in stress and defence responses and peptidases; and down-regulation of lacACDG and pyruvate formate-lyase were observed in the TY_S_NaF group, as compared to TY_S group. Conclusions: The presence of NaF has decreased the overall gene expression level inS. mutans. However, its major effect seems to be the inducing of expression of genes involved in some PEP:PTS systems and other metabolic transporters which imply specific cellular internalisation of sugars.

AB - Objective: Considering the diverse mechanisms by which fluoride could affect oral bacteria, this study evaluated the effect of sodium fluoride onStreptococcus mutans transcriptome in the presence of sucrose. Methods: S. mutans UA159 was cultured in 3 different types of media: medium control[TY], sucrose control[TY_S], and sodium fluoride sucrose test[TY_S_NaF]. Triplicates of each group were sampled at exponential phase 3 h after inoculation, total RNA was isolated, mRNA enriched and cDNA paired-end sequenced (Illumina Hi-Seq2500). Results: Genes related toS. mutans adhesion(gtfB and gtfC), acidogenicity and sugar transport were up-regulated in the presence of sucrose(TY_S) and sucrose/fluoride(TY_S_NaF), whereas gene dltA, D-alanine-activating enzyme, which is related to regulation of non-PTS sugar internalization was down-regulated. Up-regulation of the scrA gene and the PTS fructose-and mannose system, as well as functions such as those involved in stress and defence responses and peptidases; and down-regulation of lacACDG and pyruvate formate-lyase were observed in the TY_S_NaF group, as compared to TY_S group. Conclusions: The presence of NaF has decreased the overall gene expression level inS. mutans. However, its major effect seems to be the inducing of expression of genes involved in some PEP:PTS systems and other metabolic transporters which imply specific cellular internalisation of sugars.

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Streptococcus mutans transcriptome in the presence of sodium fluoride and sucrose

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