Structural and mechanical insights into DNA and chromosomes under strain using optical tweezers and fluorescence microscopy

Erwin J.G. Peterman

doi:10.1117/12.2682173

Structural and mechanical insights into DNA and chromosomes under strain using optical tweezers and fluorescence microscopy

Erwin J.G. Peterman^*

^*Corresponding author for this work

Research output: Chapter in Book / Report / Conference proceeding › Conference contribution › Academic › peer-review

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Abstract

In our lab, we have developed the combination of optical tweezers and single-molecule fluorescence microscopy as a powerful tool to study biomolecules and biomolecular complexes. Combining these two technologies allows holding a sample molecule or complex, extending or deforming it, and measuring forces acting on it, while, at the same time, visualizing it with single-molecule sensitivity. We have applied this approach to different biological systems, including DNA, whole chromosomes, cytoskeletal components and membranes. Here, I will explain the concept of the technology, its potential and limitations. I will explain the technology by highlighting its application to our research on the mechanical properties of DNA and force-induced conformational transitions. I will also discuss our latest breakthroughs in applying this technology to intact mitotic human chromosomes, which provide novel insights in the mechanical properties of chromosomes and the way they are condensed in mitosis.

Original language	English
Title of host publication	Optical Trapping and Optical Micromanipulation XX
Editors	Kishan Dholakia, Gabriel C. Spalding
Publisher	SPIE
ISBN (Electronic)	9781510665125
DOIs	https://doi.org/10.1117/12.2682173
Publication status	Published - 2023
Event	Optical Trapping and Optical Micromanipulation XX 2023 - San Diego, United States Duration: 20 Aug 2023 → 24 Aug 2023

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	12649
ISSN (Print)	0277-786X
ISSN (Electronic)	1996-756X

Conference

Conference	Optical Trapping and Optical Micromanipulation XX 2023
Country/Territory	United States
City	San Diego
Period	20/08/23 → 24/08/23

Bibliographical note

Funding Information:
I thank the people involved in my lab and those of Gijs Wuite, Iddo Heller and Chase Broedersz at Vrije Universiteit Amsterdam, and our collaborators in the lab of Ian Hickson at Copenhagen University.

Publisher Copyright:
© 2023 SPIE.

Keywords

chromosomes
DNA
DNA-binding proteins
fluorescence microscopy
Optical tweezers
single-molecule techniques

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10.1117/12.2682173

ProcSPIETemplate_A4_EP_Structural and mechanical insightsFinal published version, 353 KBLicence: Article 25fa Dutch Copyright Act

https://www.researchgate.net/publication/374500680_Structural_and_mechanical_insights_into_DNA_and_chromosomes_under_strain_using_optical_tweezers_and_fluorescence_microscopy

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Peterman, E. J. G. (2023). Structural and mechanical insights into DNA and chromosomes under strain using optical tweezers and fluorescence microscopy. In K. Dholakia, & G. C. Spalding (Eds.), Optical Trapping and Optical Micromanipulation XX Article 1264902 (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 12649). SPIE. https://doi.org/10.1117/12.2682173

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title = "Structural and mechanical insights into DNA and chromosomes under strain using optical tweezers and fluorescence microscopy",

abstract = "In our lab, we have developed the combination of optical tweezers and single-molecule fluorescence microscopy as a powerful tool to study biomolecules and biomolecular complexes. Combining these two technologies allows holding a sample molecule or complex, extending or deforming it, and measuring forces acting on it, while, at the same time, visualizing it with single-molecule sensitivity. We have applied this approach to different biological systems, including DNA, whole chromosomes, cytoskeletal components and membranes. Here, I will explain the concept of the technology, its potential and limitations. I will explain the technology by highlighting its application to our research on the mechanical properties of DNA and force-induced conformational transitions. I will also discuss our latest breakthroughs in applying this technology to intact mitotic human chromosomes, which provide novel insights in the mechanical properties of chromosomes and the way they are condensed in mitosis.",

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note = "Funding Information: I thank the people involved in my lab and those of Gijs Wuite, Iddo Heller and Chase Broedersz at Vrije Universiteit Amsterdam, and our collaborators in the lab of Ian Hickson at Copenhagen University. Publisher Copyright: {\textcopyright} 2023 SPIE.; Optical Trapping and Optical Micromanipulation XX 2023 ; Conference date: 20-08-2023 Through 24-08-2023",

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doi = "10.1117/12.2682173",

language = "English",

series = "Proceedings of SPIE - The International Society for Optical Engineering",

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Peterman, EJG 2023, Structural and mechanical insights into DNA and chromosomes under strain using optical tweezers and fluorescence microscopy. in K Dholakia & GC Spalding (eds), Optical Trapping and Optical Micromanipulation XX., 1264902, Proceedings of SPIE - The International Society for Optical Engineering, vol. 12649, SPIE, Optical Trapping and Optical Micromanipulation XX 2023, San Diego, United States, 20/08/23. https://doi.org/10.1117/12.2682173

Structural and mechanical insights into DNA and chromosomes under strain using optical tweezers and fluorescence microscopy. / Peterman, Erwin J.G.
Optical Trapping and Optical Micromanipulation XX. ed. / Kishan Dholakia; Gabriel C. Spalding. SPIE, 2023. 1264902 (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 12649).

Research output: Chapter in Book / Report / Conference proceeding › Conference contribution › Academic › peer-review

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N1 - Funding Information: I thank the people involved in my lab and those of Gijs Wuite, Iddo Heller and Chase Broedersz at Vrije Universiteit Amsterdam, and our collaborators in the lab of Ian Hickson at Copenhagen University. Publisher Copyright: © 2023 SPIE.

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N2 - In our lab, we have developed the combination of optical tweezers and single-molecule fluorescence microscopy as a powerful tool to study biomolecules and biomolecular complexes. Combining these two technologies allows holding a sample molecule or complex, extending or deforming it, and measuring forces acting on it, while, at the same time, visualizing it with single-molecule sensitivity. We have applied this approach to different biological systems, including DNA, whole chromosomes, cytoskeletal components and membranes. Here, I will explain the concept of the technology, its potential and limitations. I will explain the technology by highlighting its application to our research on the mechanical properties of DNA and force-induced conformational transitions. I will also discuss our latest breakthroughs in applying this technology to intact mitotic human chromosomes, which provide novel insights in the mechanical properties of chromosomes and the way they are condensed in mitosis.

AB - In our lab, we have developed the combination of optical tweezers and single-molecule fluorescence microscopy as a powerful tool to study biomolecules and biomolecular complexes. Combining these two technologies allows holding a sample molecule or complex, extending or deforming it, and measuring forces acting on it, while, at the same time, visualizing it with single-molecule sensitivity. We have applied this approach to different biological systems, including DNA, whole chromosomes, cytoskeletal components and membranes. Here, I will explain the concept of the technology, its potential and limitations. I will explain the technology by highlighting its application to our research on the mechanical properties of DNA and force-induced conformational transitions. I will also discuss our latest breakthroughs in applying this technology to intact mitotic human chromosomes, which provide novel insights in the mechanical properties of chromosomes and the way they are condensed in mitosis.

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Y2 - 20 August 2023 through 24 August 2023

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Structural and mechanical insights into DNA and chromosomes under strain using optical tweezers and fluorescence microscopy

Abstract

Publication series

Conference

Bibliographical note

Keywords

Access to Document

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