TY - JOUR
T1 - Structural investigation of an extracellular polysaccharide produced by the cariogenic bacterium Streptococcus mutans strain UA159
AU - Li, B.
AU - Dobruchowskab, J.M.
AU - Hoogenkamp, M.A.
AU - Gerwig, G.J.
PY - 2012
Y1 - 2012
N2 - The structure of an extracellular polysaccharide EPS159 produced from sucrose by Streptococcus mutans UA159 was investigated through the main oligosaccharides obtained from partial acid hydrolysis, monosaccharide/methylation analysis, and 1D/2D 1H NMR spectroscopy. The results showed that EPS159 contained terminal, 3-substituted, 6-substituted, and 3,6-disubstituted α-d-glucopyranose residues in a molar percentage of 14, 18, 54, and 14%. The backbone of EPS159 was composed of →6)Glcp(1→ residues, and about 20% of the →6)Glcp(1→ residues was substituted at 3-OH by →3)Glcp(1→ and/or Glcp(1→ residues to form side chains. A composite model of EPS159, that includes all identified structural features, was formulated.
AB - The structure of an extracellular polysaccharide EPS159 produced from sucrose by Streptococcus mutans UA159 was investigated through the main oligosaccharides obtained from partial acid hydrolysis, monosaccharide/methylation analysis, and 1D/2D 1H NMR spectroscopy. The results showed that EPS159 contained terminal, 3-substituted, 6-substituted, and 3,6-disubstituted α-d-glucopyranose residues in a molar percentage of 14, 18, 54, and 14%. The backbone of EPS159 was composed of →6)Glcp(1→ residues, and about 20% of the →6)Glcp(1→ residues was substituted at 3-OH by →3)Glcp(1→ and/or Glcp(1→ residues to form side chains. A composite model of EPS159, that includes all identified structural features, was formulated.
U2 - 10.1016/j.carbpol.2012.05.095
DO - 10.1016/j.carbpol.2012.05.095
M3 - Article
SN - 0008-6215
VL - 90
SP - 675
EP - 682
JO - Carbohydrate Research
JF - Carbohydrate Research
IS - 1
ER -