Structural Transitions and Energy Landscape for Cowpea Chlorotic Mottle Virus Capsid Mechanics from Nanomanipulation in Vitro and in Silico

O. Kononova, S. Snijder, M. Brasch, J. Cornelissen, R.I. Dima, K.A. Marx, G.J.L. Wuite, W.H. Roos, V. Barsegov

Research output: Contribution to JournalArticleAcademicpeer-review


Physical properties of capsids of plant and animal viruses are important factors in capsid self-assembly, survival of viruses in the extracellular environment, and their cell infectivity. Combined AFM experiments and computational modeling on subsecond timescales of the indentation nanomechanics of Cowpea Chlorotic Mottle Virus capsid show that the capsid's physical properties are dynamic and local characteristics of the structure, which change with the depth of indentation and depend on the magnitude and geometry of mechanical input. Under large deformations, the Cowpea Chlorotic Mottle Virus capsid transitions to the collapsed state without substantial local structural alterations. The enthalpy change in this deformation state ΔH
Original languageEnglish
Pages (from-to)1893-1903
JournalBiophysical Journal
Issue number8
Publication statusPublished - 2013


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