Subgingival Microbiota during Healthy Pregnancy and Pregnancy Gingivitis

P. Balan, B.W. Brandt, Y.S. Chong, W. Crielaard, M.L. Wong, V. Lopez, H.G. He, C.J. Seneviratne

Research output: Contribution to JournalArticleAcademicpeer-review

131 Downloads (Pure)

Abstract

Introduction: Previous studies have largely explored the microbial composition and pathogenesis of pregnancy gingivitis. However, the patterns of microbial colonization during pregnancy in the absence of pregnancy gingivitis have rarely been studied. Characterization of the oral microbiome in pregnant women with healthy gingiva is an important initial step in understanding the role of the microbiome in progression to pregnancy gingivitis.
Objectives: In this study, we compared the oral microbiome of pregnant women without gingivitis (healthy pregnancy) with pregnant women having gingivitis and nonpregnant healthy women to understand how pregnancy modifies the oral microbiome and induces progression to pregnancy gingivitis.
Methods: Subgingival plaque samples were collected from Chinese pregnant women with gingivitis (n = 10), healthy pregnant women (n = 10), and nonpregnant healthy women (n = 10). The Illumina MiSeq platform was used to perform 16S rRNA gene sequencing targeting the V4 region.
Results: The alpha and beta diversity was significantly different between pregnant and nonpregnant women, but minimal differences were observed between pregnant women with and without gingivitis. Interestingly, the oral bacterial community showed higher abundance of pathogenic taxa during healthy pregnancy as compared with nonpregnant women despite similar gingival and plaque index scores. However, when compared with overt pregnancy gingivitis, pathogenic taxa were less abundant during healthy pregnancy. PICRUSt analysis (phylogenetic investigation of communities by reconstruction of unobserved states) also suggested no difference in the functional capabilities of the microbiome during pregnancy, irrespective of gingival disease status. However, metabolic pathways related to amino acid metabolism were significantly increased in healthy pregnant women as compared with nonpregnant women.
Conclusion: The presence of pathogenic taxa in healthy pregnancy and pregnancy gingivitis suggests that bacteria may be necessary for initiating disease development but progression to gingivitis may be influenced by the host environmental factors. More efforts are required to plan interventions aimed at sustaining health before the appearance of overt gingivitis.
Knowledge Transfer Statement: The results of this study draw attention to the importance of oral health maintenance during pregnancy, as women without any prenatal oral conditions are predisposed to the risk of developing pregnancy gingivitis. Hence, it is important to incorporate comprehensive assessment of oral health in the prenatal health care schedules. Pregnant woman should be screened for oral risks, counseled on proper oral hygiene and expected oral changes, and referred for dental treatment, when necessary.
Original languageEnglish
Pages (from-to)343-351
Number of pages9
JournalJDR Clinical and Translational Research
Volume6
Issue number3
Early online date10 Aug 2020
DOIs
Publication statusPublished - Jul 2021

Funding

This study was supported by the Oral Health Seed Grant, Faculty of Dentistry (000-076-733), Sunstar Donation Grant (000-111-720) and SingHealth Duke NUS NDRIS grant (11/FY2019/G1/02-A44) to C.J.S.

FundersFunder number
Faculty of Dentistry000-076-733, 000-111-720
SingHealth Duke NUS11/FY2019/G1/02-A44
National University of Singapore
Faculty of Science, National University of SingaporeOral Health Seed Grant #R-221-000-076-733
SingHealth Foundation
Institute of Water Policy, National University of Singapore

    Fingerprint

    Dive into the research topics of 'Subgingival Microbiota during Healthy Pregnancy and Pregnancy Gingivitis'. Together they form a unique fingerprint.

    Cite this