TY - JOUR
T1 - Substrates for the at-line coupling of capillary electrophoresis and surface-enhanced Raman spectroscopy
AU - Dijkstra, R.J.
AU - Gerssen, A.
AU - Efremov, E.V.
AU - Ariese, F.
AU - Brinkman, U.A.T.
AU - Gooijer, C.
PY - 2004
Y1 - 2004
N2 - In this preliminary study, we evaluated four different types of substrate for the at-line coupling of capillary electrophoresis and surface-enhanced (resonance) Raman spectroscopy, CE-SER(R)S, with emphasis on spectral repeatability. We tested Sub1: etched silver foil, Sub2: a vapour-deposited silver film, Sub3: a silver oxalate-precoated silica TLC plate and Sub4: a silica TLC plate on which colloid and poly(L-lysine) were manually added to the analyte spots, used earlier in at-line CE-SE(R)RS. All substrates were first tested by manual spotting using trans-1,2-bis(4-pyridyl)ethylene (BPE) as a model compound for SERS and crystal violet (CV) as a model compound for SE(R)RS. The spectral features of the SE(R)RS spectra of BPE and CV showed a most satisfactory repeatability on all substrates. As expected, the signal intensities varied considerably between runs; this implies that quantification in at-line CE-SE(R)RS should rather be done by means of an on-line absorbance detector. In addition, the suitability of Sub1, Sub2 and Sub4 as deposition substrates after CE was explored using two cationic dyes: CV and basic fuchsin (BF). Good-quality SERRS spectra could be recorded on all three substrates. Although Sub1 and Sub2 have a poor water-sorptivity, they were found to be good substrates for at-line CE-SERRS. They do not require any post-deposition addition of silver colloid and could therefore become attractive alternatives for Sub4. © 2003 Elsevier B.V. All rights reserved.
AB - In this preliminary study, we evaluated four different types of substrate for the at-line coupling of capillary electrophoresis and surface-enhanced (resonance) Raman spectroscopy, CE-SER(R)S, with emphasis on spectral repeatability. We tested Sub1: etched silver foil, Sub2: a vapour-deposited silver film, Sub3: a silver oxalate-precoated silica TLC plate and Sub4: a silica TLC plate on which colloid and poly(L-lysine) were manually added to the analyte spots, used earlier in at-line CE-SE(R)RS. All substrates were first tested by manual spotting using trans-1,2-bis(4-pyridyl)ethylene (BPE) as a model compound for SERS and crystal violet (CV) as a model compound for SE(R)RS. The spectral features of the SE(R)RS spectra of BPE and CV showed a most satisfactory repeatability on all substrates. As expected, the signal intensities varied considerably between runs; this implies that quantification in at-line CE-SE(R)RS should rather be done by means of an on-line absorbance detector. In addition, the suitability of Sub1, Sub2 and Sub4 as deposition substrates after CE was explored using two cationic dyes: CV and basic fuchsin (BF). Good-quality SERRS spectra could be recorded on all three substrates. Although Sub1 and Sub2 have a poor water-sorptivity, they were found to be good substrates for at-line CE-SERRS. They do not require any post-deposition addition of silver colloid and could therefore become attractive alternatives for Sub4. © 2003 Elsevier B.V. All rights reserved.
U2 - 10.1016/j.aca.2003.11.066
DO - 10.1016/j.aca.2003.11.066
M3 - Article
SN - 0003-2670
VL - 508
SP - 127
EP - 134
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
IS - 2
ER -
