Supramolecular organization of photosystem II and its associated light-harvesting antenna in partially solubilized hotosystem II membranes

E.J. Boekema, H. van Roon, J.F.L. van Breemen, J.P. Dekker

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

We present an extended analysis of the organization of green plant photosystem II and its associated light-harvesting antenna using electron microscopy and image analysis. The analysis is based on a large dataset of 16 600 projections of negatively stained PSII-LHCII supercomplexes and megacomplexes prepared by means of three different pretreatments. In addition to our previous work on this system [Boekema, E.J., van Roon, H., Calkoen, F., Bassi, R. and Dekker, J.P. (1999) Biochemistry 38, 2233-2239], the following results were obtained. The rotational orientation of trimeric LHCII at the S, M and L binding positions was determined. It was found that compared to the S trimer, the M and L trimers are rotationally shifted by about -20°C and -50°C, respectively. The number of projections with empty CP29, CP26 and CP24 binding sites was found to be about 0, 18 and 4%, respectively. We suggest that CP26 and CP24 are not required for the binding of trimeric LHCII at any of the three binding positions. A new type of megacomplex was observed with a characteristic windmill-like shape. This type III megacomplex consists of two C
Original languageEnglish
Pages (from-to)444-452
JournalEuropean Journal of Biochemistry
Volume266
DOIs
Publication statusPublished - 1999

Fingerprint

Dive into the research topics of 'Supramolecular organization of photosystem II and its associated light-harvesting antenna in partially solubilized hotosystem II membranes'. Together they form a unique fingerprint.

Cite this