Switching an Individual Phycobilisome off and on

Michal Gwizdala*, Joshua L. Botha, Adjélé Wilson, Diana Kirilovsky, Rienk Van Grondelle, Tjaart P.J. Krüger

*Corresponding author for this work

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Photosynthetic organisms have found various smart ways to cope with unexpected changes in light conditions. In many cyanobacteria, the lethal effects of a sudden increase in light intensity are mitigated mainly by the interaction between phycobilisomes (PBs) and the orange carotenoid protein (OCP). The latter senses high light intensities by means of photoactivation and triggers thermal energy dissipation from the PBs. Due to the brightness of their emission, PBs can be characterized at the level of individual complexes. Here, energy dissipation from individual PBs was reversibly switched on and off using only light and OCP. We reveal the presence of quasistable intermediate states during the binding and unbinding of OCP to PB, with a spectroscopic signature indicative of transient decoupling of some of the PB rods during docking of OCP. Real-time control of emission from individual PBs has the potential to contribute to the development of new super-resolution imaging techniques.

Original languageEnglish
Pages (from-to)2426-2432
Number of pages7
JournalJournal of Physical Chemistry Letters
Volume9
Issue number9
DOIs
Publication statusPublished - 3 May 2018

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