Targeting, isertion and localization of E. coli YidC.

M.L. Urbanus, L. Froderberg, D Drew, P Bjork, J.-W. de Gier, J Brunner, B. Oudega, S. Luirink

Research output: Contribution to JournalArticleAcademic

71 Downloads (Pure)

Abstract

YidC was recently shown to play an important role in the assembly of inner membrane proteins (IMPs) both in conjunction with and separate from the Sec-translocon. Little is known about the biogenesis and structural and functional properties of YidC, itself a polytopic IMP. Here we analyze the targeting and membrane integration of YidC using in vivo and in vitro approaches. The combined data indicate that YidC is targeted by the signal recognition particle and inserts at the SecAYEG-YidC translocon early during biogenesis, unlike its mitochondrial homologue Oxalp. In addition, YidC is shown to be relatively abundant compared with other components involved in IMP assembly and is predominantly localized at the poles of the cell.
Original languageEnglish
Pages (from-to)12718-12723
Number of pages5
JournalJournal of Biological Chemistry
Volume277
DOIs
Publication statusPublished - 2002

Fingerprint

Dive into the research topics of 'Targeting, isertion and localization of E. coli YidC.'. Together they form a unique fingerprint.

Cite this