Teflubenzuron effects on springtail life history traits explained from impairment of its lipid metabolism

Diana Ilyaskina, Saúl Fernandes, Matty P. Berg, Marja H. Lamoree, Cornelis A.M. van Gestel, Pim E.G. Leonards*

*Corresponding author for this work

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

This study investigated how the insecticide teflubenzuron disrupts lipid metabolism in the springtail Folsomia candida, revealing significant alterations in lipid profiles. F. candida was exposed to sub-lethal concentrations of teflubenzuron (0, 0.006, 0.014, 0.035 mg a.s. kg−1 soil dry weight). Untargeted lipidomics was used to study the dynamic changes in lipid profiles in the springtail over exposure intervals of 2, 7, and 14 days exposure intervals. Teflubenzuron induced shifts in lipid profiles, affecting lipid pathways crucial for energy storage, membrane integrity, and signaling, which are essential for survival, reproduction, and stress responses in this springtail. Diacylglycerols (DG) and Triacylglycerols (TG), which play crucial roles in energy storage and lipid-mediated signaling, were substantially affected by teflubenzuron. Decreased levels of DG and TG suggest a shift in priorities from reproduction to maintenance functions, implying disruptions in cholesterol homeostasis and vitellogenesis in response to teflubenzuron exposure. Furthermore, increased levels of fatty acids and N-acylethanolamines in response to teflubenzuron exposure indicated increased energy production and potential oxidative stress, highlighting the springtails' response to pesticide exposure. Certain lipid alterations (N-palmitoylethanolamine (NAE 16:0) and N-stearoylethanolamine (NAE 18:0)), known for their anti-inflammatory properties, point towards inflammation and mitochondrial membrane remodeling (alternations in cardiolipin lipids), indicating broader impacts on physiological functions. Ether glycerophospholipids, such as lysophosphatidylethanolamine and phosphatidylethanolamine, linked to peroxisomes and the endoplasmic reticulum, underscore their potential antioxidative role in response to oxidative stress. The study shows the significance of incorporating life cycle events into ecotoxicological assessments to comprehensively understand pesticide impacts on organisms. The integration of lipidomics into environmental risk assessments offers a more informed approach to pesticide regulation and environmental stewardship.

Original languageEnglish
Article number179394
Pages (from-to)1-15
Number of pages15
JournalScience of the Total Environment
Volume978
Early online date18 Apr 2025
DOIs
Publication statusPublished - 25 May 2025

Bibliographical note

Publisher Copyright:
© 2025

Keywords

  • Energy storage
  • Folsomia candida
  • Insect growth regulators
  • Life cycle event
  • Lipidomics
  • Oxidative stress
  • Sub-lethal effects

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