Temporal expression profile of an accessory-gland protein that is transferred via the seminal fluid of the simultaneous hermaphrodite Lymnaea stagnalis

Elferra M Swart, Angus Davison, Jacintha Ellers, Riccardo R Filangieri, Daniel J Jackson, Janine Mariën, Isabelle B C van der Ouderaa, Dick Roelofs, Joris M Koene

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Male accessory-gland proteins are known to affect female physiology in multiple ways, maximizing a male’s reproductive success—often at a cost to the female. Due to this inherent sexual conflict, accessory gland proteins (ACPs) are generally studied in separate-sex organisms. While ACPs have also been identified in simultaneous hermaphrodites as an important part of post-copulatory sexual selection processes, their study has lagged behind that of ACPs in organisms with separate sexes. In the great pond snail, Lymnaea stagnalis, an ACP affecting egg laying, ovipostatin, is produced in the prostate gland. Based on the published partial Ovipostatin gene sequence, we now provide the complete mRNA and gene sequences, and confirm that gene expression is prostate gland-specific. More importantly we observed a significant increase in Ovipostatin expression in sperm donors after ejaculation. Ovipostatin gene expression did not differ between donors giving their ejaculate first (primary donors) and those donating an ejaculate after having been inseminated (secondary donors). These observations support a role for ovipostatin in reproduction and highlight the importance of standardizing the time point when measuring expression levels of ACPs.
Original languageEnglish
Article numbereyz005
Pages (from-to)177-183
Number of pages7
JournalJournal of Molluscan Studies
Volume85
Issue number2
Early online date26 Mar 2019
DOIs
Publication statusPublished - May 2019

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Lymnaea stagnalis
hermaphrodite
protein
fluid
prostate gland
proteins
gene expression
nucleotide sequences
sexual conflict
ejaculation
gene
gender
organisms
sexual selection
sperm
snail
physiology
snails
fluids
oviposition

Cite this

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title = "Temporal expression profile of an accessory-gland protein that is transferred via the seminal fluid of the simultaneous hermaphrodite Lymnaea stagnalis",
abstract = "Male accessory-gland proteins are known to affect female physiology in multiple ways, maximizing a male’s reproductive success—often at a cost to the female. Due to this inherent sexual conflict, accessory gland proteins (ACPs) are generally studied in separate-sex organisms. While ACPs have also been identified in simultaneous hermaphrodites as an important part of post-copulatory sexual selection processes, their study has lagged behind that of ACPs in organisms with separate sexes. In the great pond snail, Lymnaea stagnalis, an ACP affecting egg laying, ovipostatin, is produced in the prostate gland. Based on the published partial Ovipostatin gene sequence, we now provide the complete mRNA and gene sequences, and confirm that gene expression is prostate gland-specific. More importantly we observed a significant increase in Ovipostatin expression in sperm donors after ejaculation. Ovipostatin gene expression did not differ between donors giving their ejaculate first (primary donors) and those donating an ejaculate after having been inseminated (secondary donors). These observations support a role for ovipostatin in reproduction and highlight the importance of standardizing the time point when measuring expression levels of ACPs.",
author = "Swart, {Elferra M} and Angus Davison and Jacintha Ellers and Filangieri, {Riccardo R} and Jackson, {Daniel J} and Janine Mari{\"e}n and {van der Ouderaa}, {Isabelle B C} and Dick Roelofs and Koene, {Joris M}",
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Temporal expression profile of an accessory-gland protein that is transferred via the seminal fluid of the simultaneous hermaphrodite Lymnaea stagnalis. / Swart, Elferra M; Davison, Angus; Ellers, Jacintha; Filangieri, Riccardo R; Jackson, Daniel J; Mariën, Janine; van der Ouderaa, Isabelle B C; Roelofs, Dick; Koene, Joris M.

In: Journal of Molluscan Studies, Vol. 85, No. 2, eyz005, 05.2019, p. 177-183.

Research output: Contribution to JournalArticleAcademicpeer-review

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AU - Swart, Elferra M

AU - Davison, Angus

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AU - Filangieri, Riccardo R

AU - Jackson, Daniel J

AU - Mariën, Janine

AU - van der Ouderaa, Isabelle B C

AU - Roelofs, Dick

AU - Koene, Joris M

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