TY - JOUR
T1 - Temporal expression profile of an accessory-gland protein that is transferred via the seminal fluid of the simultaneous hermaphrodite Lymnaea stagnalis
AU - Swart, Elferra M
AU - Davison, Angus
AU - Ellers, Jacintha
AU - Filangieri, Riccardo R
AU - Jackson, Daniel J
AU - Mariën, Janine
AU - van der Ouderaa, Isabelle B C
AU - Roelofs, Dick
AU - Koene, Joris M
PY - 2019/5
Y1 - 2019/5
N2 - Male accessory-gland proteins are known to affect female physiology in multiple ways, maximizing a male’s reproductive success—often at a cost to the female. Due to this inherent sexual conflict, accessory gland proteins (ACPs) are generally studied in separate-sex organisms. While ACPs have also been identified in simultaneous hermaphrodites as an important part of post-copulatory sexual selection processes, their study has lagged behind that of ACPs in organisms with separate sexes. In the great pond snail, Lymnaea stagnalis, an ACP affecting egg laying, ovipostatin, is produced in the prostate gland. Based on the published partial Ovipostatin gene sequence, we now provide the complete mRNA and gene sequences, and confirm that gene expression is prostate gland-specific. More importantly we observed a significant increase in Ovipostatin expression in sperm donors after ejaculation. Ovipostatin gene expression did not differ between donors giving their ejaculate first (primary donors) and those donating an ejaculate after having been inseminated (secondary donors). These observations support a role for ovipostatin in reproduction and highlight the importance of standardizing the time point when measuring expression levels of ACPs.
AB - Male accessory-gland proteins are known to affect female physiology in multiple ways, maximizing a male’s reproductive success—often at a cost to the female. Due to this inherent sexual conflict, accessory gland proteins (ACPs) are generally studied in separate-sex organisms. While ACPs have also been identified in simultaneous hermaphrodites as an important part of post-copulatory sexual selection processes, their study has lagged behind that of ACPs in organisms with separate sexes. In the great pond snail, Lymnaea stagnalis, an ACP affecting egg laying, ovipostatin, is produced in the prostate gland. Based on the published partial Ovipostatin gene sequence, we now provide the complete mRNA and gene sequences, and confirm that gene expression is prostate gland-specific. More importantly we observed a significant increase in Ovipostatin expression in sperm donors after ejaculation. Ovipostatin gene expression did not differ between donors giving their ejaculate first (primary donors) and those donating an ejaculate after having been inseminated (secondary donors). These observations support a role for ovipostatin in reproduction and highlight the importance of standardizing the time point when measuring expression levels of ACPs.
UR - http://www.scopus.com/inward/record.url?scp=85071324792&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85071324792&partnerID=8YFLogxK
U2 - 10.1093/mollus/eyz005
DO - 10.1093/mollus/eyz005
M3 - Article
SN - 0260-1230
VL - 85
SP - 177
EP - 183
JO - Journal of Molluscan Studies
JF - Journal of Molluscan Studies
IS - 2
M1 - eyz005
ER -