The application of HPLC with on-line coupled UV/MS-biochemical detection for isolation of an acetylcholinesterase inhibitor from Narcissus 'Sir Winston Churchill'

K. Ingkaninan; A. Hazekamp; C.M. de Best; H. Irth; U.R. Tjaden; R. van der Heijden; J. van der Greef; R. Verpoorte

doi:10.1021/np9905719

The application of HPLC with on-line coupled UV/MS-biochemical detection for isolation of an acetylcholinesterase inhibitor from Narcissus 'Sir Winston Churchill'

K. Ingkaninan, A. Hazekamp, C.M. de Best, H. Irth, U.R. Tjaden, R. van der Heijden, J. van der Greef, R. Verpoorte

BioAnalytical Chemistry

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

An HPLC with on-line coupled UV/MS-biochemical detection method for acetylcholinesterase (AChE) inhibitors in natural sources has been developed. The potential of this method is shown by the isolation of a new AChE inhibitor from the alcoholic extract of Narcissus 'Sir Winston Churchill'. Combining a prefractionation technique using centrifugal partition chromatography with the on-line HPLC-UV/MS-biochemical detection resulted in the isolation of the active compound that was identified as ungiminorine. This alkaloid shows a mild inhibitory effect on AChE.

Original language	English
Pages (from-to)	803-806
Number of pages	4
Journal	Journal of Natural Products
Volume	63
Issue number	6
DOIs	https://doi.org/10.1021/np9905719
Publication status	Published - 2000

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10.1021/np9905719

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Ingkaninan, K., Hazekamp, A., de Best, C. M., Irth, H., Tjaden, U. R., van der Heijden, R., van der Greef, J., & Verpoorte, R. (2000). The application of HPLC with on-line coupled UV/MS-biochemical detection for isolation of an acetylcholinesterase inhibitor from Narcissus 'Sir Winston Churchill'. Journal of Natural Products, 63(6), 803-806. https://doi.org/10.1021/np9905719

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title = "The application of HPLC with on-line coupled UV/MS-biochemical detection for isolation of an acetylcholinesterase inhibitor from Narcissus 'Sir Winston Churchill'",

abstract = "An HPLC with on-line coupled UV/MS-biochemical detection method for acetylcholinesterase (AChE) inhibitors in natural sources has been developed. The potential of this method is shown by the isolation of a new AChE inhibitor from the alcoholic extract of Narcissus 'Sir Winston Churchill'. Combining a prefractionation technique using centrifugal partition chromatography with the on-line HPLC-UV/MS-biochemical detection resulted in the isolation of the active compound that was identified as ungiminorine. This alkaloid shows a mild inhibitory effect on AChE.",

author = "K. Ingkaninan and A. Hazekamp and {de Best}, C.M. and H. Irth and U.R. Tjaden and {van der Heijden}, R. and {van der Greef}, J. and R. Verpoorte",

year = "2000",

doi = "10.1021/np9905719",

language = "English",

volume = "63",

pages = "803--806",

journal = "Journal of Natural Products",

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Ingkaninan, K, Hazekamp, A, de Best, CM, Irth, H, Tjaden, UR, van der Heijden, R, van der Greef, J & Verpoorte, R 2000, 'The application of HPLC with on-line coupled UV/MS-biochemical detection for isolation of an acetylcholinesterase inhibitor from Narcissus 'Sir Winston Churchill'', Journal of Natural Products, vol. 63, no. 6, pp. 803-806. https://doi.org/10.1021/np9905719

TY - JOUR

T1 - The application of HPLC with on-line coupled UV/MS-biochemical detection for isolation of an acetylcholinesterase inhibitor from Narcissus 'Sir Winston Churchill'

AU - Ingkaninan, K.

AU - Hazekamp, A.

AU - de Best, C.M.

AU - Irth, H.

AU - Tjaden, U.R.

AU - van der Heijden, R.

AU - van der Greef, J.

AU - Verpoorte, R.

PY - 2000

Y1 - 2000

N2 - An HPLC with on-line coupled UV/MS-biochemical detection method for acetylcholinesterase (AChE) inhibitors in natural sources has been developed. The potential of this method is shown by the isolation of a new AChE inhibitor from the alcoholic extract of Narcissus 'Sir Winston Churchill'. Combining a prefractionation technique using centrifugal partition chromatography with the on-line HPLC-UV/MS-biochemical detection resulted in the isolation of the active compound that was identified as ungiminorine. This alkaloid shows a mild inhibitory effect on AChE.

AB - An HPLC with on-line coupled UV/MS-biochemical detection method for acetylcholinesterase (AChE) inhibitors in natural sources has been developed. The potential of this method is shown by the isolation of a new AChE inhibitor from the alcoholic extract of Narcissus 'Sir Winston Churchill'. Combining a prefractionation technique using centrifugal partition chromatography with the on-line HPLC-UV/MS-biochemical detection resulted in the isolation of the active compound that was identified as ungiminorine. This alkaloid shows a mild inhibitory effect on AChE.

U2 - 10.1021/np9905719

DO - 10.1021/np9905719

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VL - 63

SP - 803

EP - 806

JO - Journal of Natural Products

JF - Journal of Natural Products

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IS - 6

ER -

The application of HPLC with on-line coupled UV/MS-biochemical detection for isolation of an acetylcholinesterase inhibitor from Narcissus 'Sir Winston Churchill'

Abstract

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