The application of HPLC with on-line coupled UV/MS-biochemical detection for isolation of an acetylcholinesterase inhibitor from Narcissus 'Sir Winston Churchill'

K. Ingkaninan, A. Hazekamp, C.M. de Best, H. Irth, U.R. Tjaden, R. van der Heijden, J. van der Greef, R. Verpoorte

    Research output: Contribution to JournalArticleAcademicpeer-review

    Abstract

    An HPLC with on-line coupled UV/MS-biochemical detection method for acetylcholinesterase (AChE) inhibitors in natural sources has been developed. The potential of this method is shown by the isolation of a new AChE inhibitor from the alcoholic extract of Narcissus 'Sir Winston Churchill'. Combining a prefractionation technique using centrifugal partition chromatography with the on-line HPLC-UV/MS-biochemical detection resulted in the isolation of the active compound that was identified as ungiminorine. This alkaloid shows a mild inhibitory effect on AChE.
    Original languageEnglish
    Pages (from-to)803-806
    Number of pages4
    JournalJournal of Natural Products
    Volume63
    Issue number6
    DOIs
    Publication statusPublished - 2000

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