The application of HPLC with on-line coupled UV/MS-biochemical detection for isolation of an acetylcholinesterase inhibitor from Narcissus 'Sir Winston Churchill'

K. Ingkaninan, A. Hazekamp, C.M. de Best, H. Irth, U.R. Tjaden, R. van der Heijden, J. van der Greef, R. Verpoorte

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

An HPLC with on-line coupled UV/MS-biochemical detection method for acetylcholinesterase (AChE) inhibitors in natural sources has been developed. The potential of this method is shown by the isolation of a new AChE inhibitor from the alcoholic extract of Narcissus 'Sir Winston Churchill'. Combining a prefractionation technique using centrifugal partition chromatography with the on-line HPLC-UV/MS-biochemical detection resulted in the isolation of the active compound that was identified as ungiminorine. This alkaloid shows a mild inhibitory effect on AChE.
Original languageEnglish
Pages (from-to)803-806
Number of pages4
JournalJournal of Natural Products
Volume63
Issue number6
DOIs
Publication statusPublished - 2000

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