Abstract
An HPLC with on-line coupled UV/MS-biochemical detection method for acetylcholinesterase (AChE) inhibitors in natural sources has been developed. The potential of this method is shown by the isolation of a new AChE inhibitor from the alcoholic extract of Narcissus 'Sir Winston Churchill'. Combining a prefractionation technique using centrifugal partition chromatography with the on-line HPLC-UV/MS-biochemical detection resulted in the isolation of the active compound that was identified as ungiminorine. This alkaloid shows a mild inhibitory effect on AChE.
Original language | English |
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Pages (from-to) | 803-806 |
Number of pages | 4 |
Journal | Journal of Natural Products |
Volume | 63 |
Issue number | 6 |
DOIs | |
Publication status | Published - 2000 |