The application of HPLC with on-line coupled UV/MS-biochemical detection for isolation of an acetylcholinesterase inhibitor from Narcissus 'Sir Winston Churchill'

K. Ingkaninan; A. Hazekamp; C.M. de Best; H. Irth; U.R. Tjaden; R. van der Heijden; J. van der Greef; R. Verpoorte

doi:10.1021/np9905719

The application of HPLC with on-line coupled UV/MS-biochemical detection for isolation of an acetylcholinesterase inhibitor from Narcissus 'Sir Winston Churchill'

K. Ingkaninan
, A. Hazekamp
, C.M. de Best
, H. Irth
, U.R. Tjaden
, R. van der Heijden
, J. van der Greef
, R. Verpoorte

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

An HPLC with on-line coupled UV/MS-biochemical detection method for acetylcholinesterase (AChE) inhibitors in natural sources has been developed. The potential of this method is shown by the isolation of a new AChE inhibitor from the alcoholic extract of Narcissus 'Sir Winston Churchill'. Combining a prefractionation technique using centrifugal partition chromatography with the on-line HPLC-UV/MS-biochemical detection resulted in the isolation of the active compound that was identified as ungiminorine. This alkaloid shows a mild inhibitory effect on AChE.

Original language	English
Pages (from-to)	803-806
Number of pages	4
Journal	Journal of Natural Products
Volume	63
Issue number	6
DOIs	https://doi.org/10.1021/np9905719
Publication status	Published - 2000

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SDG 3 Good Health and Well-being

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10.1021/np9905719

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Ingkaninan, K., Hazekamp, A., de Best, C. M., Irth, H., Tjaden, U. R., van der Heijden, R., van der Greef, J., & Verpoorte, R. (2000). The application of HPLC with on-line coupled UV/MS-biochemical detection for isolation of an acetylcholinesterase inhibitor from Narcissus 'Sir Winston Churchill'. Journal of Natural Products, 63(6), 803-806. https://doi.org/10.1021/np9905719

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abstract = "An HPLC with on-line coupled UV/MS-biochemical detection method for acetylcholinesterase (AChE) inhibitors in natural sources has been developed. The potential of this method is shown by the isolation of a new AChE inhibitor from the alcoholic extract of Narcissus 'Sir Winston Churchill'. Combining a prefractionation technique using centrifugal partition chromatography with the on-line HPLC-UV/MS-biochemical detection resulted in the isolation of the active compound that was identified as ungiminorine. This alkaloid shows a mild inhibitory effect on AChE.",

author = "K. Ingkaninan and A. Hazekamp and \{de Best\}, C.M. and H. Irth and U.R. Tjaden and \{van der Heijden\}, R. and \{van der Greef\}, J. and R. Verpoorte",

year = "2000",

doi = "10.1021/np9905719",

language = "English",

volume = "63",

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journal = "Journal of Natural Products",

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Ingkaninan, K, Hazekamp, A, de Best, CM, Irth, H, Tjaden, UR, van der Heijden, R, van der Greef, J & Verpoorte, R 2000, 'The application of HPLC with on-line coupled UV/MS-biochemical detection for isolation of an acetylcholinesterase inhibitor from Narcissus 'Sir Winston Churchill'', Journal of Natural Products, vol. 63, no. 6, pp. 803-806. https://doi.org/10.1021/np9905719

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T1 - The application of HPLC with on-line coupled UV/MS-biochemical detection for isolation of an acetylcholinesterase inhibitor from Narcissus 'Sir Winston Churchill'

AU - Ingkaninan, K.

AU - Hazekamp, A.

AU - de Best, C.M.

AU - Irth, H.

AU - Tjaden, U.R.

AU - van der Heijden, R.

AU - van der Greef, J.

AU - Verpoorte, R.

PY - 2000

Y1 - 2000

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AB - An HPLC with on-line coupled UV/MS-biochemical detection method for acetylcholinesterase (AChE) inhibitors in natural sources has been developed. The potential of this method is shown by the isolation of a new AChE inhibitor from the alcoholic extract of Narcissus 'Sir Winston Churchill'. Combining a prefractionation technique using centrifugal partition chromatography with the on-line HPLC-UV/MS-biochemical detection resulted in the isolation of the active compound that was identified as ungiminorine. This alkaloid shows a mild inhibitory effect on AChE.

U2 - 10.1021/np9905719

DO - 10.1021/np9905719

M3 - Article

SN - 0163-3864

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IS - 6

ER -

The application of HPLC with on-line coupled UV/MS-biochemical detection for isolation of an acetylcholinesterase inhibitor from Narcissus 'Sir Winston Churchill'

Abstract

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