TY - JOUR
T1 - The bactericidal effect of a Genius Nd: YAG laser
AU - Kranendonk, A.A.
AU - van der Reijden, W.A.
AU - van Winkelhoff, A.J.
AU - van der Weijden, G.A.
PY - 2010
Y1 - 2010
N2 - Purpose: To evaluate the ‘in vitro’ bactericidal effect of the Nd:YAG laser (Genius, MØlsgaard Dental, Copenhagen, Denmark) on six periodontal pathogens. Methods: Suspensions of six different periodontal pathogens (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, Fusobacterium nucleatum and Parvimonas micra) were prepared in small Eppendorff tubes, and exposed to a Nd:YAG laser for five different periods of time. Laser settings used: Power 6 Watt (on a scale of 1-12 W), Frequency 50 Hz, Pulse duration 250 μs. After exposure to the laser, aliquots of the suspensions were spread on blood agar plates for bacterial counting. Results: After 5 s of laser exposure, there was a decrease in total colony forming units for all six selected microorganisms. After 15, 30 and 45 s, no viable bacterial cells could be retrieved. Conclusion: In this ‘in vitro’ model, 15 s of Nd:YAG laser use was found to be effective for total killing of the six tested periodontal pathogens.
AB - Purpose: To evaluate the ‘in vitro’ bactericidal effect of the Nd:YAG laser (Genius, MØlsgaard Dental, Copenhagen, Denmark) on six periodontal pathogens. Methods: Suspensions of six different periodontal pathogens (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, Fusobacterium nucleatum and Parvimonas micra) were prepared in small Eppendorff tubes, and exposed to a Nd:YAG laser for five different periods of time. Laser settings used: Power 6 Watt (on a scale of 1-12 W), Frequency 50 Hz, Pulse duration 250 μs. After exposure to the laser, aliquots of the suspensions were spread on blood agar plates for bacterial counting. Results: After 5 s of laser exposure, there was a decrease in total colony forming units for all six selected microorganisms. After 15, 30 and 45 s, no viable bacterial cells could be retrieved. Conclusion: In this ‘in vitro’ model, 15 s of Nd:YAG laser use was found to be effective for total killing of the six tested periodontal pathogens.
U2 - 10.1111/j.1601-5037.2009.00375.x
DO - 10.1111/j.1601-5037.2009.00375.x
M3 - Article
SN - 1601-5029
VL - 8
SP - 63
EP - 67
JO - International Journal of Dental Hygiene
JF - International Journal of Dental Hygiene
IS - 1
ER -