The bioenergetics of denitrification

A.H. Stouthamer, F C Boogerd, H W van Verseveld

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

In anaerobically grown Paracoccus denitrificans the dissimilatory nitrate reductase is linked to the respiratory chain at the level of cytochromes b. Electron transport to nitrite and nitrous oxide involves c-type cytochromes. During electron transport from NADH to nitrate one phosphorylation site is passed, whereas two sites are passed during electron transport from NADH to oxygen, nitrite and nitrous oxide. The presentation of a respiratory chain as a linear array of electron carriers gives a misleading picture of the efficiency of energy conservation since the location of the reductases is not taken into account. For the reduction of nitrite and nitrous oxide, protons are utilized from the periplasmic space, whereas for the reduction of oxygen and nitrate, protons are utilized from the cytoplasmic side of the inner membrane. Evidence for two transport systems for nitrate was obtained. One is driven by the proton motive force; this system is used to initiate nitrate reduction. The second system is a nitrate-nitrite antiport system. A scheme for proton translocation and electron transport to nitrate, nitrite, nitrous oxide and oxygen is presented. The number of charges translocated across the membrane during flow of two electrons from NADH is the same for all nitrogenous oxides and is 67-71% of that during electron transfer to oxygen via cytochrome o. These findings are in accordance with growth yield studies. YMAX electron values determined in chemostat cultures for growth with various substrates and hydrogen acceptors are proportional to the number of charges translocated to these hydrogen acceptors during electron transport.

Original languageEnglish
Pages (from-to)545-53
Number of pages9
JournalAntonie van Leeuwenhoek
Volume48
Issue number6
Publication statusPublished - 1982

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Denitrification
Electron Transport
Energy Metabolism
Nitrates
Nitrites
Nitrous Oxide
NAD
Electrons
Oxygen
Protons
Hydrogen
Paracoccus denitrificans
Cytochrome c Group
Nitrate Reductase
Periplasm
Proton-Motive Force
Cytochromes b
Membranes
Ion Transport
Cytochromes

Keywords

  • Adenosine Triphosphate
  • Biological Transport, Active
  • Cytochromes
  • Electron Transport
  • Energy Metabolism
  • Hydrogen
  • NAD
  • Nitrates
  • Nitrites
  • Nitrogen
  • Nitrous Oxide
  • Oxidation-Reduction
  • Oxidative Phosphorylation
  • Paracoccus denitrificans
  • Pseudomonas
  • Journal Article
  • Research Support, Non-U.S. Gov't
  • Review

Cite this

Stouthamer, A. H., Boogerd, F. C., & van Verseveld, H. W. (1982). The bioenergetics of denitrification. Antonie van Leeuwenhoek, 48(6), 545-53.
Stouthamer, A.H. ; Boogerd, F C ; van Verseveld, H W. / The bioenergetics of denitrification. In: Antonie van Leeuwenhoek. 1982 ; Vol. 48, No. 6. pp. 545-53.
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Stouthamer, AH, Boogerd, FC & van Verseveld, HW 1982, 'The bioenergetics of denitrification' Antonie van Leeuwenhoek, vol. 48, no. 6, pp. 545-53.

The bioenergetics of denitrification. / Stouthamer, A.H.; Boogerd, F C; van Verseveld, H W.

In: Antonie van Leeuwenhoek, Vol. 48, No. 6, 1982, p. 545-53.

Research output: Contribution to JournalArticleAcademicpeer-review

TY - JOUR

T1 - The bioenergetics of denitrification

AU - Stouthamer, A.H.

AU - Boogerd, F C

AU - van Verseveld, H W

PY - 1982

Y1 - 1982

N2 - In anaerobically grown Paracoccus denitrificans the dissimilatory nitrate reductase is linked to the respiratory chain at the level of cytochromes b. Electron transport to nitrite and nitrous oxide involves c-type cytochromes. During electron transport from NADH to nitrate one phosphorylation site is passed, whereas two sites are passed during electron transport from NADH to oxygen, nitrite and nitrous oxide. The presentation of a respiratory chain as a linear array of electron carriers gives a misleading picture of the efficiency of energy conservation since the location of the reductases is not taken into account. For the reduction of nitrite and nitrous oxide, protons are utilized from the periplasmic space, whereas for the reduction of oxygen and nitrate, protons are utilized from the cytoplasmic side of the inner membrane. Evidence for two transport systems for nitrate was obtained. One is driven by the proton motive force; this system is used to initiate nitrate reduction. The second system is a nitrate-nitrite antiport system. A scheme for proton translocation and electron transport to nitrate, nitrite, nitrous oxide and oxygen is presented. The number of charges translocated across the membrane during flow of two electrons from NADH is the same for all nitrogenous oxides and is 67-71% of that during electron transfer to oxygen via cytochrome o. These findings are in accordance with growth yield studies. YMAX electron values determined in chemostat cultures for growth with various substrates and hydrogen acceptors are proportional to the number of charges translocated to these hydrogen acceptors during electron transport.

AB - In anaerobically grown Paracoccus denitrificans the dissimilatory nitrate reductase is linked to the respiratory chain at the level of cytochromes b. Electron transport to nitrite and nitrous oxide involves c-type cytochromes. During electron transport from NADH to nitrate one phosphorylation site is passed, whereas two sites are passed during electron transport from NADH to oxygen, nitrite and nitrous oxide. The presentation of a respiratory chain as a linear array of electron carriers gives a misleading picture of the efficiency of energy conservation since the location of the reductases is not taken into account. For the reduction of nitrite and nitrous oxide, protons are utilized from the periplasmic space, whereas for the reduction of oxygen and nitrate, protons are utilized from the cytoplasmic side of the inner membrane. Evidence for two transport systems for nitrate was obtained. One is driven by the proton motive force; this system is used to initiate nitrate reduction. The second system is a nitrate-nitrite antiport system. A scheme for proton translocation and electron transport to nitrate, nitrite, nitrous oxide and oxygen is presented. The number of charges translocated across the membrane during flow of two electrons from NADH is the same for all nitrogenous oxides and is 67-71% of that during electron transfer to oxygen via cytochrome o. These findings are in accordance with growth yield studies. YMAX electron values determined in chemostat cultures for growth with various substrates and hydrogen acceptors are proportional to the number of charges translocated to these hydrogen acceptors during electron transport.

KW - Adenosine Triphosphate

KW - Biological Transport, Active

KW - Cytochromes

KW - Electron Transport

KW - Energy Metabolism

KW - Hydrogen

KW - NAD

KW - Nitrates

KW - Nitrites

KW - Nitrogen

KW - Nitrous Oxide

KW - Oxidation-Reduction

KW - Oxidative Phosphorylation

KW - Paracoccus denitrificans

KW - Pseudomonas

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

KW - Review

M3 - Article

VL - 48

SP - 545

EP - 553

JO - Antonie van Leeuwenhoek

JF - Antonie van Leeuwenhoek

SN - 0003-6072

IS - 6

ER -

Stouthamer AH, Boogerd FC, van Verseveld HW. The bioenergetics of denitrification. Antonie van Leeuwenhoek. 1982;48(6):545-53.