The carcinoGENOMICS project: critical selection of model compounds for the development of omics-based in vitro carcinogenicity screening assays

Mathieu Vinken, Tatyana Doktorova, Heidrun Ellinger-Ziegelbauer, Hans-Jürgen Ahr, Edward A Lock, Paul Carmichael, Erwin Roggen, Joost H van Delft, Jos Kleinjans, José Castell, Roque Bort, Teresa Donato, Michael P Ryan, Raffaella Corvi, Hector C Keun, Timothy Mark David Ebbels, Toby J Athersuch, Susanna-Assunta Sansone, Philippe Rocca-Serra, Rob StierumPaul Jennings, Walter Pfaller, Hans Gmuender, Tamara Vanhaecke, Vera Rogiers

Research output: Contribution to JournalReview articleAcademicpeer-review

Abstract

Recent changes in the European legislation of chemical-related substances have forced the scientific community to speed up the search for alternative methods that could partly or fully replace animal experimentation. The Sixth Framework Program project carcinoGENOMICS was specifically raised to develop omics-based in vitro screens for testing the carcinogenic potential of chemical compounds in a pan-European context. This paper provides an in-depth analysis of the complexity of choosing suitable reference compounds used for creating and fine-tuning the in vitro carcinogenicity assays. First, a number of solid criteria for the selection of the model compounds are defined. Secondly, the strategy followed, including resources consulted, is described and the selected compounds are briefly illustrated. Finally, limitations and problems encountered during the selection procedure are discussed. Since selecting an appropriate set of chemicals is a frequent impediment in the early stages of similar research projects, the information provided in this paper might be extremely valuable.

Original languageEnglish
Pages (from-to)202-10
Number of pages9
JournalMutation Research/Reviews in Mutation Research
Volume659
Issue number3
DOIs
Publication statusPublished - 3 Jun 2008

Keywords

  • Animal Testing Alternatives
  • Carcinogenicity Tests
  • European Union
  • Gene Expression Profiling
  • Genomics
  • Hazardous Substances
  • International Cooperation
  • Toxicogenetics
  • Journal Article
  • Research Support, Non-U.S. Gov't
  • Review

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