The conserved third transmembrane segment of YidC contacts nascent Escherichia coli inner membrane proteins.

Z. Yu, G.M. Koningstein, O.I. Pop, S. Luirink

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    Abstract

    Escherichia coli YidC is a polytopic inner membrane protein that plays an essential and versatile role in the biogenesis of inner membrane proteins. YidC functions in Sec-dependent membrane insertion but acts also independently as a separate insertase for certain small membrane proteins. We have used a site-specific cross-linking approach to show that the conserved third transmembrane segment of YidC contacts the transmembrane domains of both nascent Sec-dependent and -independent substrates, indicating a generic recognition of insertion intermediates by YidC. Our data suggest that specific residues of the third YidC transmembrane segment α-helix is oriented toward the transmembrane domains of nascent inner membrane proteins that, in contrast, appear quite flexibly positioned at this stage in biogenesis. © 2008 by The American Society for Biochemistry and Molecular Biology, Inc.
    Original languageEnglish
    JournalJournal of Biological Chemistry
    DOIs
    Publication statusPublished - 2009

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