The E. coli ERP and SecB targeting pathway converge at the translocon

Q.A. Valent, P.A. Scotti, S. High, J-W.L de Gier, G. von Heijne, G. Lentzen, W. Wintermeyer, B. Oudega, S. Luirink

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Two distinct protein targeting pathways can direct proteins to the Escherichia coli inner membrane. The Sec pathway involves the cytosolic chaperone SecB that binds to the mature region of pre-proteins. SecB targets the pre-protein to SecA that mediates preprotein translocation through the SecYEG translocon. The SRP pathway is probably used primarily for the targeting and assembly of inner membrane proteins. It involves the signal recognition particle (SRP) that interacts with the hydrophobic targeting signal of nascent proteins. By using a protein cross-linking approach, we demonstrate here that the SRP pathway delivers nascent inner membrane proteins at the membrane. The SRP receptor FtsY, GTP and inner membranes are required for release of the nascent proteins from the SRP. Upon release of the SRP at the membrane, the targeted nascent proteins insert into a translocon that contains at least SecA, SecY and SecG. Hence, as appears to be the case for several other translocation systems, multiple targeting mechanisms deliver a variety of precursor proteins to a common membrane translocation complex of the E.coli inner membrane.
Original languageEnglish
Pages (from-to)2504-2512
Number of pages9
JournalEMBO Journal
Volume17
DOIs
Publication statusPublished - 1998

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Signal Recognition Particle
Enterprise resource planning
Escherichia coli
Membranes
Proteins
Membrane Proteins
Protein Precursors
Escherichia coli Proteins
Protein Transport
Guanosine Triphosphate
Die casting inserts

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Valent, Q. A., Scotti, P. A., High, S., de Gier, J-W. L., von Heijne, G., Lentzen, G., ... Luirink, S. (1998). The E. coli ERP and SecB targeting pathway converge at the translocon. EMBO Journal, 17, 2504-2512. https://doi.org/10.1093/emboj/17.9.2504
Valent, Q.A. ; Scotti, P.A. ; High, S. ; de Gier, J-W.L ; von Heijne, G. ; Lentzen, G. ; Wintermeyer, W. ; Oudega, B. ; Luirink, S. / The E. coli ERP and SecB targeting pathway converge at the translocon. In: EMBO Journal. 1998 ; Vol. 17. pp. 2504-2512.
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title = "The E. coli ERP and SecB targeting pathway converge at the translocon",
abstract = "Two distinct protein targeting pathways can direct proteins to the Escherichia coli inner membrane. The Sec pathway involves the cytosolic chaperone SecB that binds to the mature region of pre-proteins. SecB targets the pre-protein to SecA that mediates preprotein translocation through the SecYEG translocon. The SRP pathway is probably used primarily for the targeting and assembly of inner membrane proteins. It involves the signal recognition particle (SRP) that interacts with the hydrophobic targeting signal of nascent proteins. By using a protein cross-linking approach, we demonstrate here that the SRP pathway delivers nascent inner membrane proteins at the membrane. The SRP receptor FtsY, GTP and inner membranes are required for release of the nascent proteins from the SRP. Upon release of the SRP at the membrane, the targeted nascent proteins insert into a translocon that contains at least SecA, SecY and SecG. Hence, as appears to be the case for several other translocation systems, multiple targeting mechanisms deliver a variety of precursor proteins to a common membrane translocation complex of the E.coli inner membrane.",
author = "Q.A. Valent and P.A. Scotti and S. High and {de Gier}, J-W.L and {von Heijne}, G. and G. Lentzen and W. Wintermeyer and B. Oudega and S. Luirink",
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Valent, QA, Scotti, PA, High, S, de Gier, J-WL, von Heijne, G, Lentzen, G, Wintermeyer, W, Oudega, B & Luirink, S 1998, 'The E. coli ERP and SecB targeting pathway converge at the translocon' EMBO Journal, vol. 17, pp. 2504-2512. https://doi.org/10.1093/emboj/17.9.2504

The E. coli ERP and SecB targeting pathway converge at the translocon. / Valent, Q.A.; Scotti, P.A.; High, S.; de Gier, J-W.L; von Heijne, G.; Lentzen, G.; Wintermeyer, W.; Oudega, B.; Luirink, S.

In: EMBO Journal, Vol. 17, 1998, p. 2504-2512.

Research output: Contribution to JournalArticleAcademicpeer-review

TY - JOUR

T1 - The E. coli ERP and SecB targeting pathway converge at the translocon

AU - Valent, Q.A.

AU - Scotti, P.A.

AU - High, S.

AU - de Gier, J-W.L

AU - von Heijne, G.

AU - Lentzen, G.

AU - Wintermeyer, W.

AU - Oudega, B.

AU - Luirink, S.

PY - 1998

Y1 - 1998

N2 - Two distinct protein targeting pathways can direct proteins to the Escherichia coli inner membrane. The Sec pathway involves the cytosolic chaperone SecB that binds to the mature region of pre-proteins. SecB targets the pre-protein to SecA that mediates preprotein translocation through the SecYEG translocon. The SRP pathway is probably used primarily for the targeting and assembly of inner membrane proteins. It involves the signal recognition particle (SRP) that interacts with the hydrophobic targeting signal of nascent proteins. By using a protein cross-linking approach, we demonstrate here that the SRP pathway delivers nascent inner membrane proteins at the membrane. The SRP receptor FtsY, GTP and inner membranes are required for release of the nascent proteins from the SRP. Upon release of the SRP at the membrane, the targeted nascent proteins insert into a translocon that contains at least SecA, SecY and SecG. Hence, as appears to be the case for several other translocation systems, multiple targeting mechanisms deliver a variety of precursor proteins to a common membrane translocation complex of the E.coli inner membrane.

AB - Two distinct protein targeting pathways can direct proteins to the Escherichia coli inner membrane. The Sec pathway involves the cytosolic chaperone SecB that binds to the mature region of pre-proteins. SecB targets the pre-protein to SecA that mediates preprotein translocation through the SecYEG translocon. The SRP pathway is probably used primarily for the targeting and assembly of inner membrane proteins. It involves the signal recognition particle (SRP) that interacts with the hydrophobic targeting signal of nascent proteins. By using a protein cross-linking approach, we demonstrate here that the SRP pathway delivers nascent inner membrane proteins at the membrane. The SRP receptor FtsY, GTP and inner membranes are required for release of the nascent proteins from the SRP. Upon release of the SRP at the membrane, the targeted nascent proteins insert into a translocon that contains at least SecA, SecY and SecG. Hence, as appears to be the case for several other translocation systems, multiple targeting mechanisms deliver a variety of precursor proteins to a common membrane translocation complex of the E.coli inner membrane.

U2 - 10.1093/emboj/17.9.2504

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M3 - Article

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SP - 2504

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JO - EMBO Journal

JF - EMBO Journal

SN - 0261-4189

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Valent QA, Scotti PA, High S, de Gier J-WL, von Heijne G, Lentzen G et al. The E. coli ERP and SecB targeting pathway converge at the translocon. EMBO Journal. 1998;17:2504-2512. https://doi.org/10.1093/emboj/17.9.2504