The effect of a single injection of irinotecan on the development of enamel in the Wistar rats

S. Al-Ansari, R. Jalali, T. Bronckers, J. Raber-Durlacher, R. Logan, J. de Lange, F. Rozema

Research output: Contribution to JournalArticleAcademicpeer-review


Cancer is the second most frequent cause of death in children. Because the prognosis for childhood malignancies has improved, attention has now focused on long-term consequences of cancer treatment. The immediate effects of chemotherapy on soft tissues have been well described; however, there is less information about long-term effects of chemotherapy on the development of dental tissues. To test the association between the effect of chemotherapy on enamel development, we examined two groups of rats: one that had received an intraperitoneal dose of 200 mg/kg of irinotecan, whereas the other (control) group had received vehicle only. Rats were killed at 6, 48 and 96 hr post-injection; the mandibles dissected out, fixed for histological evaluation and scanned for mineralization defects by Micro-CT. Our results showed structural changes in the ameloblast layer along with a significant reduction in mineralization and thickness of enamel at 96 hr after chemotherapy. These data demonstrate that irinotecan induces structural changes in forming enamel that become apparent after anticancer chemotherapy treatment.
Original languageEnglish
Pages (from-to)1501-1506
JournalJournal of Cellular and Molecular Medicine
Issue number3
Publication statusPublished - 2018


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