Abstract
INTRODUCTION: The aim of the study was to evaluate the modulating effects of five commonly used sweetener (glucose, inulin, isomaltulose, tagatose, trehalose) containing mouth rinses on the oral microbiome.
METHODS: A single-centre, double-blind, parallel randomized clinical trial was performed with healthy, 18-55-year-old volunteers (N = 65), who rinsed thrice-daily for two weeks with a 10% solution of one of the allocated sweeteners. Microbiota composition of supragingival dental plaque and the tongue dorsum coating was analysed by 16S RNA gene amplicon sequencing of the V4 hypervariable region (Illumina MiSeq). As secondary outcomes, dental plaque red fluorescence and salivary pH were measured.
RESULTS: Dental plaque microbiota changed significantly for two groups: inulin (F = 2.0239, p = 0.0006 PERMANOVA, Aitchison distance) and isomaltulose (F = 0.67, p = 0.0305). For the tongue microbiota, significant changes were observed for isomaltulose (F = 0.8382, p = 0.0452) and trehalose (F = 1.0119, p = 0.0098). In plaque, 13 species changed significantly for the inulin group, while for tongue coating, three species changed for the trehalose group (ALDEx2, p < 0.1). No significant changes were observed for the secondary outcomes.
CONCLUSION: The effects on the oral microbiota were sweetener dependant with the most pronounced effect on plaque microbiota. Inulin exhibited the strongest microbial modulating potential of the sweeteners tested. Further full-scale clinical studies are required.
| Original language | English |
|---|---|
| Article number | 2369350 |
| Pages (from-to) | 1-15 |
| Number of pages | 15 |
| Journal | Journal of Oral Microbiology |
| Volume | 16 |
| Issue number | 1 |
| Early online date | 24 Jun 2024 |
| DOIs | |
| Publication status | Published - 2024 |
Bibliographical note
© 2024 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.Funding
The collaboration project between ACTA and TNO was co-funded by the PPP Allowance made available by Health~Holland, Top Sector Life Sciences & Health, to stimulate public-private partnerships. The project was further co-funded by GSK and Philips. Additional support was received from Ministry of Economic Affairs and Climate Policy (The Netherlands). DWK gratefully acknowledges the University of Amsterdam Data Science Centre for financial support provided for this project. We would like to express our sincere appreciation and gratitude to Wendy (W.E.A.J.) de Wit, Michel (M.A.) Hoogenkamp, Rob (R.A.M.) Exterkate, Mark (M.J.) Buijs, Dono (D.) Kahharova, Danuta (D.) Mazurel, Elly (E.C.) van Deutekom-Mulder, Carolien (C.J.) Bosch-Tijhof, Pauline (P.S.) Ouwerling, Maud (W.) Pullens, Sytze (S.P.) Ouwerling, Najib (N.) Sawo and Ilona (I.F.) Persoon for contributing to the successful completion of this clinical study.
| Funders | Funder number |
|---|---|
| Philips | |
| University of Amsterdam Data Science Centre | |
| GlaxoSmithKline | |
| Health~Holland, Top Sector Life Sciences & Health | |
| Ministerie van Economische Zaken en Klimaat |