The effect of EMG processing choices on muscle synergies before and after BoNT-A treatment in cerebral palsy

Marjolein M. van der Krogt, Laura Oudenhoven, Annemieke I. Buizer, Annet Dallmeijer, Nadia Dominici, Jaap Harlaar

Research output: Contribution to ConferencePosterOther research output

Abstract

Introduction: Recently, the use of synergy analysis to describe dynamic motor control in cerebral palsy (CP) has become increasingly popular, particularly because synergies have been shown to be a strong predictor of treatment outcome [1]. However, several studies have shown that technical issues such as the matrix factorization algorithms used [2] and EMG processing [3,4] can influence the outcome and interpretation of synergies. Therefore, it is important to study how processing steps can influence synergy outcome measures in CP, especially before and after treatment. Research question: What is the effect of different choices in the EMG analysis (filtering, normalization, and stride selection) on the outcome of synergy analysis in CP, and could this affect the comparison of synergies before and after botulinum neurotoxin (BoNT-A) treatment? Methods: 68 ambulant children with CP (age 9.3 AE 3.6, GMFCS level I–IV) underwent multilevel BoNT-A treatment, and received pre-and 73 AE 25 days post-treatment gait analysis, with EMG measurement of 5 muscles (rectus fem., vastus lat., semitendinosus, tibialis ant. and gastrocnemius med.). EMG data of the most affected leg were high-pass filtered at 20 Hz, rectified, and low-pass filtered at either 2, 3, 10 or 25 Hz. EMG data were either not normalized, normalized to their own maximum, or normalized to the pre-treatment maximum. Finally, EMG was either averaged over all strides (N = 10.8 AE 4.2), or taken from one random stride, or concatenated over all strides. EMG with each combination of filter, normalization, and stride selection was input to a non-negative matrix factorization algorithm (NNMF). An ANOVA for repeated measures was run on the variance accounted for (VAF) by one synergy, to analyse BoNT-A treatment, filtering, normalization, and stride selection effects, as well as their interactions. Results: VAF decreased strongly with increasing filter frequency (Fig. A, p <0.001); and when including individual strides rather than the mean (Fig. B, p <0.001). No main effect of normalization was found. VAF increased slightly after BoNT-A treatment (Effect size 0.010; p = 0.044), but only when higher filter frequencies were used (Fig. A, interaction p <0.001). Discussion: Choices in EMG processing have a large effect on synergy outcomes, which can lead to conflicting findings in terms of treatment outcome. The decrease in dynamic motor control (increased VAF) after BoNT-A treatment, which was largest at higher filtering frequencies, may be due to lower amplitudes and hence reduced signal-to-noise ratio after treatment due to denervated muscles, rather than an actual change in dynamic motor control. This study shows that synergy outcomes strongly depend on EMG processing method, and may help in making deliberate choices during data analysis.
Original languageEnglish
Pages31
Number of pages1
DOIs
Publication statusPublished - 2016
EventESMAC 2016 - NH Collection Hotel, Seville, Spain
Duration: 29 Sep 20161 Oct 2016
Conference number: 25
http://esmac2016.com/

Conference

ConferenceESMAC 2016
Abbreviated titleESMAC 2016
CountrySpain
CitySeville
Period29/09/161/10/16
Internet address

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Cerebral Palsy
Muscles
Therapeutics
Ants
Neurotoxins
Signal-To-Noise Ratio
incobotulinumtoxinA
Gait
Leg
Analysis of Variance
Outcome Assessment (Health Care)
Research

Cite this

van der Krogt, M. M., Oudenhoven, L., Buizer, A. I., Dallmeijer, A., Dominici, N., & Harlaar, J. (2016). The effect of EMG processing choices on muscle synergies before and after BoNT-A treatment in cerebral palsy. 31. Poster session presented at ESMAC 2016, Seville, Spain. https://doi.org/10.1016/j.gaitpost.2016.07.095
van der Krogt, Marjolein M. ; Oudenhoven, Laura ; Buizer, Annemieke I. ; Dallmeijer, Annet ; Dominici, Nadia ; Harlaar, Jaap. / The effect of EMG processing choices on muscle synergies before and after BoNT-A treatment in cerebral palsy. Poster session presented at ESMAC 2016, Seville, Spain.1 p.
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abstract = "Introduction: Recently, the use of synergy analysis to describe dynamic motor control in cerebral palsy (CP) has become increasingly popular, particularly because synergies have been shown to be a strong predictor of treatment outcome [1]. However, several studies have shown that technical issues such as the matrix factorization algorithms used [2] and EMG processing [3,4] can influence the outcome and interpretation of synergies. Therefore, it is important to study how processing steps can influence synergy outcome measures in CP, especially before and after treatment. Research question: What is the effect of different choices in the EMG analysis (filtering, normalization, and stride selection) on the outcome of synergy analysis in CP, and could this affect the comparison of synergies before and after botulinum neurotoxin (BoNT-A) treatment? Methods: 68 ambulant children with CP (age 9.3 AE 3.6, GMFCS level I–IV) underwent multilevel BoNT-A treatment, and received pre-and 73 AE 25 days post-treatment gait analysis, with EMG measurement of 5 muscles (rectus fem., vastus lat., semitendinosus, tibialis ant. and gastrocnemius med.). EMG data of the most affected leg were high-pass filtered at 20 Hz, rectified, and low-pass filtered at either 2, 3, 10 or 25 Hz. EMG data were either not normalized, normalized to their own maximum, or normalized to the pre-treatment maximum. Finally, EMG was either averaged over all strides (N = 10.8 AE 4.2), or taken from one random stride, or concatenated over all strides. EMG with each combination of filter, normalization, and stride selection was input to a non-negative matrix factorization algorithm (NNMF). An ANOVA for repeated measures was run on the variance accounted for (VAF) by one synergy, to analyse BoNT-A treatment, filtering, normalization, and stride selection effects, as well as their interactions. Results: VAF decreased strongly with increasing filter frequency (Fig. A, p <0.001); and when including individual strides rather than the mean (Fig. B, p <0.001). No main effect of normalization was found. VAF increased slightly after BoNT-A treatment (Effect size 0.010; p = 0.044), but only when higher filter frequencies were used (Fig. A, interaction p <0.001). Discussion: Choices in EMG processing have a large effect on synergy outcomes, which can lead to conflicting findings in terms of treatment outcome. The decrease in dynamic motor control (increased VAF) after BoNT-A treatment, which was largest at higher filtering frequencies, may be due to lower amplitudes and hence reduced signal-to-noise ratio after treatment due to denervated muscles, rather than an actual change in dynamic motor control. This study shows that synergy outcomes strongly depend on EMG processing method, and may help in making deliberate choices during data analysis.",
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van der Krogt, MM, Oudenhoven, L, Buizer, AI, Dallmeijer, A, Dominici, N & Harlaar, J 2016, 'The effect of EMG processing choices on muscle synergies before and after BoNT-A treatment in cerebral palsy' ESMAC 2016, Seville, Spain, 29/09/16 - 1/10/16, pp. 31. https://doi.org/10.1016/j.gaitpost.2016.07.095

The effect of EMG processing choices on muscle synergies before and after BoNT-A treatment in cerebral palsy. / van der Krogt, Marjolein M.; Oudenhoven, Laura; Buizer, Annemieke I.; Dallmeijer, Annet; Dominici, Nadia; Harlaar, Jaap.

2016. 31 Poster session presented at ESMAC 2016, Seville, Spain.

Research output: Contribution to ConferencePosterOther research output

TY - CONF

T1 - The effect of EMG processing choices on muscle synergies before and after BoNT-A treatment in cerebral palsy

AU - van der Krogt, Marjolein M.

AU - Oudenhoven, Laura

AU - Buizer, Annemieke I.

AU - Dallmeijer, Annet

AU - Dominici, Nadia

AU - Harlaar, Jaap

PY - 2016

Y1 - 2016

N2 - Introduction: Recently, the use of synergy analysis to describe dynamic motor control in cerebral palsy (CP) has become increasingly popular, particularly because synergies have been shown to be a strong predictor of treatment outcome [1]. However, several studies have shown that technical issues such as the matrix factorization algorithms used [2] and EMG processing [3,4] can influence the outcome and interpretation of synergies. Therefore, it is important to study how processing steps can influence synergy outcome measures in CP, especially before and after treatment. Research question: What is the effect of different choices in the EMG analysis (filtering, normalization, and stride selection) on the outcome of synergy analysis in CP, and could this affect the comparison of synergies before and after botulinum neurotoxin (BoNT-A) treatment? Methods: 68 ambulant children with CP (age 9.3 AE 3.6, GMFCS level I–IV) underwent multilevel BoNT-A treatment, and received pre-and 73 AE 25 days post-treatment gait analysis, with EMG measurement of 5 muscles (rectus fem., vastus lat., semitendinosus, tibialis ant. and gastrocnemius med.). EMG data of the most affected leg were high-pass filtered at 20 Hz, rectified, and low-pass filtered at either 2, 3, 10 or 25 Hz. EMG data were either not normalized, normalized to their own maximum, or normalized to the pre-treatment maximum. Finally, EMG was either averaged over all strides (N = 10.8 AE 4.2), or taken from one random stride, or concatenated over all strides. EMG with each combination of filter, normalization, and stride selection was input to a non-negative matrix factorization algorithm (NNMF). An ANOVA for repeated measures was run on the variance accounted for (VAF) by one synergy, to analyse BoNT-A treatment, filtering, normalization, and stride selection effects, as well as their interactions. Results: VAF decreased strongly with increasing filter frequency (Fig. A, p <0.001); and when including individual strides rather than the mean (Fig. B, p <0.001). No main effect of normalization was found. VAF increased slightly after BoNT-A treatment (Effect size 0.010; p = 0.044), but only when higher filter frequencies were used (Fig. A, interaction p <0.001). Discussion: Choices in EMG processing have a large effect on synergy outcomes, which can lead to conflicting findings in terms of treatment outcome. The decrease in dynamic motor control (increased VAF) after BoNT-A treatment, which was largest at higher filtering frequencies, may be due to lower amplitudes and hence reduced signal-to-noise ratio after treatment due to denervated muscles, rather than an actual change in dynamic motor control. This study shows that synergy outcomes strongly depend on EMG processing method, and may help in making deliberate choices during data analysis.

AB - Introduction: Recently, the use of synergy analysis to describe dynamic motor control in cerebral palsy (CP) has become increasingly popular, particularly because synergies have been shown to be a strong predictor of treatment outcome [1]. However, several studies have shown that technical issues such as the matrix factorization algorithms used [2] and EMG processing [3,4] can influence the outcome and interpretation of synergies. Therefore, it is important to study how processing steps can influence synergy outcome measures in CP, especially before and after treatment. Research question: What is the effect of different choices in the EMG analysis (filtering, normalization, and stride selection) on the outcome of synergy analysis in CP, and could this affect the comparison of synergies before and after botulinum neurotoxin (BoNT-A) treatment? Methods: 68 ambulant children with CP (age 9.3 AE 3.6, GMFCS level I–IV) underwent multilevel BoNT-A treatment, and received pre-and 73 AE 25 days post-treatment gait analysis, with EMG measurement of 5 muscles (rectus fem., vastus lat., semitendinosus, tibialis ant. and gastrocnemius med.). EMG data of the most affected leg were high-pass filtered at 20 Hz, rectified, and low-pass filtered at either 2, 3, 10 or 25 Hz. EMG data were either not normalized, normalized to their own maximum, or normalized to the pre-treatment maximum. Finally, EMG was either averaged over all strides (N = 10.8 AE 4.2), or taken from one random stride, or concatenated over all strides. EMG with each combination of filter, normalization, and stride selection was input to a non-negative matrix factorization algorithm (NNMF). An ANOVA for repeated measures was run on the variance accounted for (VAF) by one synergy, to analyse BoNT-A treatment, filtering, normalization, and stride selection effects, as well as their interactions. Results: VAF decreased strongly with increasing filter frequency (Fig. A, p <0.001); and when including individual strides rather than the mean (Fig. B, p <0.001). No main effect of normalization was found. VAF increased slightly after BoNT-A treatment (Effect size 0.010; p = 0.044), but only when higher filter frequencies were used (Fig. A, interaction p <0.001). Discussion: Choices in EMG processing have a large effect on synergy outcomes, which can lead to conflicting findings in terms of treatment outcome. The decrease in dynamic motor control (increased VAF) after BoNT-A treatment, which was largest at higher filtering frequencies, may be due to lower amplitudes and hence reduced signal-to-noise ratio after treatment due to denervated muscles, rather than an actual change in dynamic motor control. This study shows that synergy outcomes strongly depend on EMG processing method, and may help in making deliberate choices during data analysis.

U2 - 10.1016/j.gaitpost.2016.07.095

DO - 10.1016/j.gaitpost.2016.07.095

M3 - Poster

SP - 31

ER -

van der Krogt MM, Oudenhoven L, Buizer AI, Dallmeijer A, Dominici N, Harlaar J. The effect of EMG processing choices on muscle synergies before and after BoNT-A treatment in cerebral palsy. 2016. Poster session presented at ESMAC 2016, Seville, Spain. https://doi.org/10.1016/j.gaitpost.2016.07.095