The hierarchy of sugar catabolization in Lactococcus cremoris

Sieze Douwenga; Berdien van Olst; Sjef Boeren; Yanzhang Luo; Xin Lai; Bas Teusink; Jacques Vervoort; Michiel Kleerebezem; Herwig Bachmann

doi:10.1128/spectrum.02248-23

The hierarchy of sugar catabolization in Lactococcus cremoris

Sieze Douwenga
, Berdien van Olst
, Sjef Boeren
, Yanzhang Luo
, Xin Lai
, Bas Teusink
, Jacques Vervoort
, Michiel Kleerebezem
, Herwig Bachmann

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

IMPORTANCE: The availability of nutrients to microorganisms varies considerably between different environments, and changes can occur rapidly. As a general rule, a fast growth rate-typically growth on glucose-is associated with the repression of other carbohydrate utilization genes, but it is not clear to what extent catabolite repression is exerted by other sugars. We investigated the hierarchy of sugar utilization after substrate transitions in Lactococcus cremoris. For this, we determined the proteome and carbohydrate utilization capacity after growth on different sugars. The results show that the preparedness of cells for the utilization of "slower" sugars is not strictly determined by the growth rate. The data point to individual proteins relevant for various sugar transitions and suggest that the evolutionary history of the organism might be responsible for deviations from a strictly growth rate-related sugar catabolization hierarchy.

Original language	English
Article number	e0224823
Pages (from-to)	1-22
Number of pages	22
Journal	Microbiology spectrum
Volume	11
Issue number	6
Early online date	27 Oct 2023
DOIs	https://doi.org/10.1128/spectrum.02248-23
Publication status	Published - Dec 2023

Funding

The project is organized and executed under the auspices of TiFN, a public-private partnership on precompetitive research in food and nutrition. H.B. is part-time employed by NIZO Food Research. The authors have declared that no competing interests exist in the writing of this publication. Funding for this research was obtained from Friesland Campina (Wageningen, The Netherlands), CSK Food Enrichment (Wageningen, The Netherlands), and Top-sector Agri&Food. This study was funded by the Top Institute Food and Nutrition (TIFN, Program 16MF01, Wageningen, The Netherlands).

Funders
Friesland Campina
Top Institute Food and Nutrition
Top-sector Agri&Food
Centralsjukhuset Kristianstad

Keywords

catabolism
catabolite repression
Lactococcus
proteome
sugar transition

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10.1128/spectrum.02248-23

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title = "The hierarchy of sugar catabolization in Lactococcus cremoris",

abstract = "IMPORTANCE: The availability of nutrients to microorganisms varies considerably between different environments, and changes can occur rapidly. As a general rule, a fast growth rate-typically growth on glucose-is associated with the repression of other carbohydrate utilization genes, but it is not clear to what extent catabolite repression is exerted by other sugars. We investigated the hierarchy of sugar utilization after substrate transitions in Lactococcus cremoris. For this, we determined the proteome and carbohydrate utilization capacity after growth on different sugars. The results show that the preparedness of cells for the utilization of {"}slower{"} sugars is not strictly determined by the growth rate. The data point to individual proteins relevant for various sugar transitions and suggest that the evolutionary history of the organism might be responsible for deviations from a strictly growth rate-related sugar catabolization hierarchy.",

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AU - Douwenga, Sieze

AU - van Olst, Berdien

AU - Boeren, Sjef

AU - Luo, Yanzhang

AU - Lai, Xin

AU - Teusink, Bas

AU - Vervoort, Jacques

AU - Kleerebezem, Michiel

AU - Bachmann, Herwig

PY - 2023/12

Y1 - 2023/12

N2 - IMPORTANCE: The availability of nutrients to microorganisms varies considerably between different environments, and changes can occur rapidly. As a general rule, a fast growth rate-typically growth on glucose-is associated with the repression of other carbohydrate utilization genes, but it is not clear to what extent catabolite repression is exerted by other sugars. We investigated the hierarchy of sugar utilization after substrate transitions in Lactococcus cremoris. For this, we determined the proteome and carbohydrate utilization capacity after growth on different sugars. The results show that the preparedness of cells for the utilization of "slower" sugars is not strictly determined by the growth rate. The data point to individual proteins relevant for various sugar transitions and suggest that the evolutionary history of the organism might be responsible for deviations from a strictly growth rate-related sugar catabolization hierarchy.

AB - IMPORTANCE: The availability of nutrients to microorganisms varies considerably between different environments, and changes can occur rapidly. As a general rule, a fast growth rate-typically growth on glucose-is associated with the repression of other carbohydrate utilization genes, but it is not clear to what extent catabolite repression is exerted by other sugars. We investigated the hierarchy of sugar utilization after substrate transitions in Lactococcus cremoris. For this, we determined the proteome and carbohydrate utilization capacity after growth on different sugars. The results show that the preparedness of cells for the utilization of "slower" sugars is not strictly determined by the growth rate. The data point to individual proteins relevant for various sugar transitions and suggest that the evolutionary history of the organism might be responsible for deviations from a strictly growth rate-related sugar catabolization hierarchy.

KW - catabolism

KW - catabolite repression

KW - Lactococcus

KW - proteome

KW - sugar transition

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The hierarchy of sugar catabolization in Lactococcus cremoris

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Keywords

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