The importance of validating the demethylating effect of 5-aza-2-deoxycytidine in model species: (A comment on Cook et al., “DNA methylation and sex allocation in the parasitoid wasp Nasonia vitripennis”)

Jacintha Ellers, Michael Visser, Janine Mariën, Ken Kraaijeveld, Mark Lammers

Research output: Contribution to JournalComment / Letter to the editorAcademic

Abstract

The use of DNA demethylating agents has been popular in epigenetic studies. Recently, Cook and colleagues, in a 2015 American Naturalist article, claimed an effect of 5-aza-2'-deoxycytidine (5-aza-dC) on the sex ratio of a parasitoid wasp without verifying its effect on DNA methylation. We repeated the 5-aza-dC feeding treatment to test its effectiveness. We used bisulfite amplicon sequencing of 10 genes that either were heavily methylated, previously showed a response to 5-aza-dC, or were suggested to regulate fatty acid synthesis epigenetically, and we demonstrate that wasps fed 5-aza-dC did not show reduced DNA methylation at these loci. Therefore, the conclusion that demethylation shifts sex ratios upward needs reconsideration.

Original languageEnglish
Pages (from-to)422-431
Number of pages10
JournalAmerican Naturalist
Volume194
Issue number3
Early online date9 Jul 2019
DOIs
Publication statusPublished - Sep 2019

Fingerprint

Nasonia vitripennis
sex allocation
methylation
DNA methylation
wasp
parasitoid
sex ratio
DNA
bisulfites
epigenetics
fatty acids
loci
synthesis
fatty acid
genes
gene
testing
parasitic wasps
effect

Keywords

  • Bisulfite amplicon sequencing
  • DNA methylation
  • Fatty acid synthesis
  • Nasonia vitripennis
  • Parasitoid
  • Sex ratio

Cite this

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title = "The importance of validating the demethylating effect of 5-aza-2′-deoxycytidine in model species: (A comment on Cook et al., “DNA methylation and sex allocation in the parasitoid wasp Nasonia vitripennis”)",
abstract = "The use of DNA demethylating agents has been popular in epigenetic studies. Recently, Cook and colleagues, in a 2015 American Naturalist article, claimed an effect of 5-aza-2'-deoxycytidine (5-aza-dC) on the sex ratio of a parasitoid wasp without verifying its effect on DNA methylation. We repeated the 5-aza-dC feeding treatment to test its effectiveness. We used bisulfite amplicon sequencing of 10 genes that either were heavily methylated, previously showed a response to 5-aza-dC, or were suggested to regulate fatty acid synthesis epigenetically, and we demonstrate that wasps fed 5-aza-dC did not show reduced DNA methylation at these loci. Therefore, the conclusion that demethylation shifts sex ratios upward needs reconsideration.",
keywords = "Bisulfite amplicon sequencing, DNA methylation, Fatty acid synthesis, Nasonia vitripennis, Parasitoid, Sex ratio",
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The importance of validating the demethylating effect of 5-aza-2-deoxycytidine in model species : (A comment on Cook et al., “DNA methylation and sex allocation in the parasitoid wasp Nasonia vitripennis”). / Ellers, Jacintha; Visser, Michael; Mariën, Janine; Kraaijeveld, Ken; Lammers, Mark.

In: American Naturalist, Vol. 194, No. 3, 09.2019, p. 422-431.

Research output: Contribution to JournalComment / Letter to the editorAcademic

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T1 - The importance of validating the demethylating effect of 5-aza-2′-deoxycytidine in model species

T2 - (A comment on Cook et al., “DNA methylation and sex allocation in the parasitoid wasp Nasonia vitripennis”)

AU - Ellers, Jacintha

AU - Visser, Michael

AU - Mariën, Janine

AU - Kraaijeveld, Ken

AU - Lammers, Mark

PY - 2019/9

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N2 - The use of DNA demethylating agents has been popular in epigenetic studies. Recently, Cook and colleagues, in a 2015 American Naturalist article, claimed an effect of 5-aza-2'-deoxycytidine (5-aza-dC) on the sex ratio of a parasitoid wasp without verifying its effect on DNA methylation. We repeated the 5-aza-dC feeding treatment to test its effectiveness. We used bisulfite amplicon sequencing of 10 genes that either were heavily methylated, previously showed a response to 5-aza-dC, or were suggested to regulate fatty acid synthesis epigenetically, and we demonstrate that wasps fed 5-aza-dC did not show reduced DNA methylation at these loci. Therefore, the conclusion that demethylation shifts sex ratios upward needs reconsideration.

AB - The use of DNA demethylating agents has been popular in epigenetic studies. Recently, Cook and colleagues, in a 2015 American Naturalist article, claimed an effect of 5-aza-2'-deoxycytidine (5-aza-dC) on the sex ratio of a parasitoid wasp without verifying its effect on DNA methylation. We repeated the 5-aza-dC feeding treatment to test its effectiveness. We used bisulfite amplicon sequencing of 10 genes that either were heavily methylated, previously showed a response to 5-aza-dC, or were suggested to regulate fatty acid synthesis epigenetically, and we demonstrate that wasps fed 5-aza-dC did not show reduced DNA methylation at these loci. Therefore, the conclusion that demethylation shifts sex ratios upward needs reconsideration.

KW - Bisulfite amplicon sequencing

KW - DNA methylation

KW - Fatty acid synthesis

KW - Nasonia vitripennis

KW - Parasitoid

KW - Sex ratio

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