Abstract
The use of DNA demethylating agents has been popular in epigenetic studies. Recently, Cook and colleagues, in a 2015 American Naturalist article, claimed an effect of 5-aza-2'-deoxycytidine (5-aza-dC) on the sex ratio of a parasitoid wasp without verifying its effect on DNA methylation. We repeated the 5-aza-dC feeding treatment to test its effectiveness. We used bisulfite amplicon sequencing of 10 genes that either were heavily methylated, previously showed a response to 5-aza-dC, or were suggested to regulate fatty acid synthesis epigenetically, and we demonstrate that wasps fed 5-aza-dC did not show reduced DNA methylation at these loci. Therefore, the conclusion that demethylation shifts sex ratios upward needs reconsideration.
Original language | English |
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Pages (from-to) | 422-431 |
Number of pages | 10 |
Journal | American Naturalist |
Volume | 194 |
Issue number | 3 |
Early online date | 9 Jul 2019 |
DOIs |
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Publication status | Published - Sept 2019 |
Keywords
- Bisulfite amplicon sequencing
- DNA methylation
- Fatty acid synthesis
- Nasonia vitripennis
- Parasitoid
- Sex ratio
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Dive into the research topics of 'The importance of validating the demethylating effect of 5-aza-2′-deoxycytidine in model species: (A comment on Cook et al., “DNA methylation and sex allocation in the parasitoid wasp Nasonia vitripennis”)'. Together they form a unique fingerprint.Datasets
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Data from: The importance of validating the demethylating effect of 5-aza-dC in model species
Ellers, J. (Contributor), Visser, M. (Contributor), Marien, J. (Contributor), Kraaijeveld, K. (Contributor) & Lammers, M. (Contributor), Unknown Publisher, 1 Jan 2019
DOI: 10.5061/dryad.d305qm1, http://datadryad.org/stash/dataset/doi:10.5061/dryad.d305qm1
Dataset