The influence of ebselen on the toxicity of cisplatin in LLC-PK1 cells

G S Baldew; A P Boymans; J G Mol; N P Vermeulen

doi:10.1016/0006-2952(92)90024-D

The influence of ebselen on the toxicity of cisplatin in LLC-PK1 cells

G S Baldew, A P Boymans, J G Mol, N P Vermeulen

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

LLC-PK1 cells have been used as an in vitro model to study the nephrotoxicity of the antitumor drug cisplatin. A concentration-dependent cytotoxicity of cisplatin, measured as lactate dehydrogenase leakage and amount of protein remaining attached to the culture plate, was observed. At a cisplatin concentration of 0.4 mM cell viability was reduced to 21% after 72 hr. Ebselen, a seleno-organic compound capable of forming selenol intermediates through reaction with thiols, was found to protect LLC-PK1 cells against cisplatin-induced toxicity at low concentrations (5-15 microM). The ebselen-induced protection against cisplatin toxicity in this in vitro test system apparently correlates well with a similar protection previously observed in vivo in mice and rats.

Original language	English
Pages (from-to)	382-7
Number of pages	6
Journal	Biochemical Pharmacology
Volume	44
Issue number	2
DOIs	https://doi.org/10.1016/0006-2952(92)90024-D
Publication status	Published - 22 Jul 1992

Keywords

Animals
Azoles
Cell Line
Cell Survival
Cisplatin
Dose-Response Relationship, Drug
Drug Interactions
Kidney
L-Lactate Dehydrogenase
Organoselenium Compounds
Rats
Swine
Time Factors
Comparative Study
Journal Article

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10.1016/0006-2952(92)90024-D

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title = "The influence of ebselen on the toxicity of cisplatin in LLC-PK1 cells",

abstract = "LLC-PK1 cells have been used as an in vitro model to study the nephrotoxicity of the antitumor drug cisplatin. A concentration-dependent cytotoxicity of cisplatin, measured as lactate dehydrogenase leakage and amount of protein remaining attached to the culture plate, was observed. At a cisplatin concentration of 0.4 mM cell viability was reduced to 21% after 72 hr. Ebselen, a seleno-organic compound capable of forming selenol intermediates through reaction with thiols, was found to protect LLC-PK1 cells against cisplatin-induced toxicity at low concentrations (5-15 microM). The ebselen-induced protection against cisplatin toxicity in this in vitro test system apparently correlates well with a similar protection previously observed in vivo in mice and rats.",

keywords = "Animals, Azoles, Cell Line, Cell Survival, Cisplatin, Dose-Response Relationship, Drug, Drug Interactions, Kidney, L-Lactate Dehydrogenase, Organoselenium Compounds, Rats, Swine, Time Factors, Comparative Study, Journal Article",

author = "Baldew, {G S} and Boymans, {A P} and Mol, {J G} and Vermeulen, {N P}",

year = "1992",

month = jul,

day = "22",

doi = "10.1016/0006-2952(92)90024-D",

language = "English",

volume = "44",

pages = "382--7",

journal = "Biochemical Pharmacology",

issn = "0006-2952",

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TY - JOUR

T1 - The influence of ebselen on the toxicity of cisplatin in LLC-PK1 cells

AU - Baldew, G S

AU - Boymans, A P

AU - Mol, J G

AU - Vermeulen, N P

PY - 1992/7/22

Y1 - 1992/7/22

N2 - LLC-PK1 cells have been used as an in vitro model to study the nephrotoxicity of the antitumor drug cisplatin. A concentration-dependent cytotoxicity of cisplatin, measured as lactate dehydrogenase leakage and amount of protein remaining attached to the culture plate, was observed. At a cisplatin concentration of 0.4 mM cell viability was reduced to 21% after 72 hr. Ebselen, a seleno-organic compound capable of forming selenol intermediates through reaction with thiols, was found to protect LLC-PK1 cells against cisplatin-induced toxicity at low concentrations (5-15 microM). The ebselen-induced protection against cisplatin toxicity in this in vitro test system apparently correlates well with a similar protection previously observed in vivo in mice and rats.

AB - LLC-PK1 cells have been used as an in vitro model to study the nephrotoxicity of the antitumor drug cisplatin. A concentration-dependent cytotoxicity of cisplatin, measured as lactate dehydrogenase leakage and amount of protein remaining attached to the culture plate, was observed. At a cisplatin concentration of 0.4 mM cell viability was reduced to 21% after 72 hr. Ebselen, a seleno-organic compound capable of forming selenol intermediates through reaction with thiols, was found to protect LLC-PK1 cells against cisplatin-induced toxicity at low concentrations (5-15 microM). The ebselen-induced protection against cisplatin toxicity in this in vitro test system apparently correlates well with a similar protection previously observed in vivo in mice and rats.

KW - Animals

KW - Azoles

KW - Cell Line

KW - Cell Survival

KW - Cisplatin

KW - Dose-Response Relationship, Drug

KW - Drug Interactions

KW - Kidney

KW - L-Lactate Dehydrogenase

KW - Organoselenium Compounds

KW - Rats

KW - Swine

KW - Time Factors

KW - Comparative Study

KW - Journal Article

U2 - 10.1016/0006-2952(92)90024-D

DO - 10.1016/0006-2952(92)90024-D

M3 - Article

C2 - 1642650

VL - 44

SP - 382

EP - 387

JO - Biochemical Pharmacology

JF - Biochemical Pharmacology

SN - 0006-2952

IS - 2

ER -

The influence of ebselen on the toxicity of cisplatin in LLC-PK1 cells

Abstract

Keywords

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