Abstract
LLC-PK1 cells have been used as an in vitro model to study the nephrotoxicity of the antitumor drug cisplatin. A concentration-dependent cytotoxicity of cisplatin, measured as lactate dehydrogenase leakage and amount of protein remaining attached to the culture plate, was observed. At a cisplatin concentration of 0.4 mM cell viability was reduced to 21% after 72 hr. Ebselen, a seleno-organic compound capable of forming selenol intermediates through reaction with thiols, was found to protect LLC-PK1 cells against cisplatin-induced toxicity at low concentrations (5-15 microM). The ebselen-induced protection against cisplatin toxicity in this in vitro test system apparently correlates well with a similar protection previously observed in vivo in mice and rats.
Original language | English |
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Pages (from-to) | 382-7 |
Number of pages | 6 |
Journal | Biochemical Pharmacology |
Volume | 44 |
Issue number | 2 |
DOIs | |
Publication status | Published - 22 Jul 1992 |
Keywords
- Animals
- Azoles
- Cell Line
- Cell Survival
- Cisplatin
- Dose-Response Relationship, Drug
- Drug Interactions
- Kidney
- L-Lactate Dehydrogenase
- Organoselenium Compounds
- Rats
- Swine
- Time Factors
- Comparative Study
- Journal Article