The influence of ebselen on the toxicity of cisplatin in LLC-PK1 cells

G S Baldew, A P Boymans, J G Mol, N P Vermeulen

Research output: Contribution to JournalArticleAcademicpeer-review


LLC-PK1 cells have been used as an in vitro model to study the nephrotoxicity of the antitumor drug cisplatin. A concentration-dependent cytotoxicity of cisplatin, measured as lactate dehydrogenase leakage and amount of protein remaining attached to the culture plate, was observed. At a cisplatin concentration of 0.4 mM cell viability was reduced to 21% after 72 hr. Ebselen, a seleno-organic compound capable of forming selenol intermediates through reaction with thiols, was found to protect LLC-PK1 cells against cisplatin-induced toxicity at low concentrations (5-15 microM). The ebselen-induced protection against cisplatin toxicity in this in vitro test system apparently correlates well with a similar protection previously observed in vivo in mice and rats.

Original languageEnglish
Pages (from-to)382-7
Number of pages6
JournalBiochemical Pharmacology
Issue number2
Publication statusPublished - 22 Jul 1992


  • Animals
  • Azoles
  • Cell Line
  • Cell Survival
  • Cisplatin
  • Dose-Response Relationship, Drug
  • Drug Interactions
  • Kidney
  • L-Lactate Dehydrogenase
  • Organoselenium Compounds
  • Rats
  • Swine
  • Time Factors
  • Comparative Study
  • Journal Article


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