The influence of long-term copper contaminated agricultural soil at different pH levels on microbial communities and springtail transcriptional regulation.

T.E. de Boer, N. Tas, M. Braster, E.J.M Temminghoff, W.F.M. Roling, D. Roelofs

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Copper has long been applied for agricultural practises. Like other metals, copper is highly persistent in the environment and biologically active long after its use has ceased. Here we present a unique study on the long-term effects (27 years) of copper and pH on soil microbial communities and on the springtail Folsomia candida an important representative of the soil macrofauna, in an experiment with a full factorial, random block design. Bacterial communities were mostly affected by pH. These effects were prominent in Acidobacteria, while Actinobacteria and Gammaroteobacteria communities were affected by original and bioavailable copper. Reproduction and survival of the collembolan F. candida was not affected by the studied copper concentrations. However, the transcriptomic responses to copper reflected a mechanism of copper transport and detoxification, while pH exerted effects on nucleotide and protein metabolism and (acute) inflammatory response. We conclude that microbial community structure reflected the history of copper contamination, while gene expression analysis of F. candida is associated with the current level of bioavailable copper. The study is a first step in the development of a molecular strategy aiming at a more comprehensive assessment of various aspects of soil quality and ecotoxicology. © 2011 American Chemical Society.
LanguageEnglish
Pages60-68
JournalEnvironmental Science and Technology
Volume46
DOIs
Publication statusPublished - 2012

Fingerprint

Collembola
agricultural soils
polluted soils
microbial communities
copper
Folsomia candida
Acidobacteria
ecotoxicology
Actinobacteria
protein metabolism
transcriptomics
bacterial communities
long term effects
soil
soil quality
community structure
inflammation
nucleotides
metals
gene expression

Cite this

@article{3fae9a4a65314ddcbd815ae497d0b883,
title = "The influence of long-term copper contaminated agricultural soil at different pH levels on microbial communities and springtail transcriptional regulation.",
abstract = "Copper has long been applied for agricultural practises. Like other metals, copper is highly persistent in the environment and biologically active long after its use has ceased. Here we present a unique study on the long-term effects (27 years) of copper and pH on soil microbial communities and on the springtail Folsomia candida an important representative of the soil macrofauna, in an experiment with a full factorial, random block design. Bacterial communities were mostly affected by pH. These effects were prominent in Acidobacteria, while Actinobacteria and Gammaroteobacteria communities were affected by original and bioavailable copper. Reproduction and survival of the collembolan F. candida was not affected by the studied copper concentrations. However, the transcriptomic responses to copper reflected a mechanism of copper transport and detoxification, while pH exerted effects on nucleotide and protein metabolism and (acute) inflammatory response. We conclude that microbial community structure reflected the history of copper contamination, while gene expression analysis of F. candida is associated with the current level of bioavailable copper. The study is a first step in the development of a molecular strategy aiming at a more comprehensive assessment of various aspects of soil quality and ecotoxicology. {\circledC} 2011 American Chemical Society.",
author = "{de Boer}, T.E. and N. Tas and M. Braster and E.J.M Temminghoff and W.F.M. Roling and D. Roelofs",
year = "2012",
doi = "10.1021/es2013598",
language = "English",
volume = "46",
pages = "60--68",
journal = "Environmental Science and Technology",
issn = "0013-936X",
publisher = "American Chemical Society",

}

The influence of long-term copper contaminated agricultural soil at different pH levels on microbial communities and springtail transcriptional regulation. / de Boer, T.E.; Tas, N.; Braster, M.; Temminghoff, E.J.M; Roling, W.F.M.; Roelofs, D.

In: Environmental Science and Technology, Vol. 46, 2012, p. 60-68.

Research output: Contribution to JournalArticleAcademicpeer-review

TY - JOUR

T1 - The influence of long-term copper contaminated agricultural soil at different pH levels on microbial communities and springtail transcriptional regulation.

AU - de Boer, T.E.

AU - Tas, N.

AU - Braster, M.

AU - Temminghoff, E.J.M

AU - Roling, W.F.M.

AU - Roelofs, D.

PY - 2012

Y1 - 2012

N2 - Copper has long been applied for agricultural practises. Like other metals, copper is highly persistent in the environment and biologically active long after its use has ceased. Here we present a unique study on the long-term effects (27 years) of copper and pH on soil microbial communities and on the springtail Folsomia candida an important representative of the soil macrofauna, in an experiment with a full factorial, random block design. Bacterial communities were mostly affected by pH. These effects were prominent in Acidobacteria, while Actinobacteria and Gammaroteobacteria communities were affected by original and bioavailable copper. Reproduction and survival of the collembolan F. candida was not affected by the studied copper concentrations. However, the transcriptomic responses to copper reflected a mechanism of copper transport and detoxification, while pH exerted effects on nucleotide and protein metabolism and (acute) inflammatory response. We conclude that microbial community structure reflected the history of copper contamination, while gene expression analysis of F. candida is associated with the current level of bioavailable copper. The study is a first step in the development of a molecular strategy aiming at a more comprehensive assessment of various aspects of soil quality and ecotoxicology. © 2011 American Chemical Society.

AB - Copper has long been applied for agricultural practises. Like other metals, copper is highly persistent in the environment and biologically active long after its use has ceased. Here we present a unique study on the long-term effects (27 years) of copper and pH on soil microbial communities and on the springtail Folsomia candida an important representative of the soil macrofauna, in an experiment with a full factorial, random block design. Bacterial communities were mostly affected by pH. These effects were prominent in Acidobacteria, while Actinobacteria and Gammaroteobacteria communities were affected by original and bioavailable copper. Reproduction and survival of the collembolan F. candida was not affected by the studied copper concentrations. However, the transcriptomic responses to copper reflected a mechanism of copper transport and detoxification, while pH exerted effects on nucleotide and protein metabolism and (acute) inflammatory response. We conclude that microbial community structure reflected the history of copper contamination, while gene expression analysis of F. candida is associated with the current level of bioavailable copper. The study is a first step in the development of a molecular strategy aiming at a more comprehensive assessment of various aspects of soil quality and ecotoxicology. © 2011 American Chemical Society.

U2 - 10.1021/es2013598

DO - 10.1021/es2013598

M3 - Article

VL - 46

SP - 60

EP - 68

JO - Environmental Science and Technology

T2 - Environmental Science and Technology

JF - Environmental Science and Technology

SN - 0013-936X

ER -