TY - JOUR
T1 - The molluscan RING-finger protein L-TRIM is essential for neuronal outgrowth
AU - van Diepen, M.T.
AU - Spencer, G.E.
AU - van Minnen, J.
AU - Gouwenberg, Y.
AU - Bouwman, J.
AU - Smit, A.B.
AU - van Kesteren, R.E.
PY - 2005/5
Y1 - 2005/5
N2 - The tripartite motif proteins TRIM-2 and TRIM-3 have been put forward as putative organizers of neuronal outgrowth and structural plasticity. Here, we identified a molluscan orthologue of TRIM-2/3, named L-TRIM, which is up-regulated during in vitro neurite outgrowth of central neurons. In adult animals, L-Trim mRNA is ubiquitously expressed at low levels in the central nervous system and in peripheral tissues. Central nervous system expression of L-Trim mRNA is increased during postnatal brain development and during in vitro and in vivo neuronal regeneration. In vitro double-stranded RNA knock-down of L-Trim mRNA resulted in a >70% inhibition of neurite outgrowth. Together, our data establish a crucial role for L-TRIM in developmental neurite outgrowth and functional neuronal regeneration and indicate that TRIM-2/3 family members may have evolutionary conserved functions in neuronal differentiation. © 2005 Elsevier Inc. All rights reserved.
AB - The tripartite motif proteins TRIM-2 and TRIM-3 have been put forward as putative organizers of neuronal outgrowth and structural plasticity. Here, we identified a molluscan orthologue of TRIM-2/3, named L-TRIM, which is up-regulated during in vitro neurite outgrowth of central neurons. In adult animals, L-Trim mRNA is ubiquitously expressed at low levels in the central nervous system and in peripheral tissues. Central nervous system expression of L-Trim mRNA is increased during postnatal brain development and during in vitro and in vivo neuronal regeneration. In vitro double-stranded RNA knock-down of L-Trim mRNA resulted in a >70% inhibition of neurite outgrowth. Together, our data establish a crucial role for L-TRIM in developmental neurite outgrowth and functional neuronal regeneration and indicate that TRIM-2/3 family members may have evolutionary conserved functions in neuronal differentiation. © 2005 Elsevier Inc. All rights reserved.
UR - https://www.scopus.com/pages/publications/18044391527
UR - https://www.scopus.com/inward/citedby.url?scp=18044391527&partnerID=8YFLogxK
U2 - 10.1016/j.mcn.2005.01.005
DO - 10.1016/j.mcn.2005.01.005
M3 - Article
SN - 1044-7431
VL - 29
SP - 74
EP - 81
JO - Molecular and Cellular Neuroscience
JF - Molecular and Cellular Neuroscience
IS - 1
ER -
