Abstract
In extracts of acid treated molybdenum cofactor containing xanthine oxidase, fluorescence is maximally developed upon a three hours incubation. Analysis by means of reversed phase HPLC revealed the presence of several fluorescent compounds, the main one being a blue fluorescent compound with an emission maximum of 465 nm when maximal excited at 395 nm at a neutral pH. Definite proof is presented that this compound is the oxidation product of the molybdenum cofactor. The remaining fluorescent products are shown to be pterin-derivatives, yielding predominantly pterin-6-carboxylic acid upon permanganate oxidation. Purified oxidation product of molybdenum cofactor however, didn's yield a fluorescent derivative at all upon treatment with permanganate.
| Original language | English |
|---|---|
| Pages (from-to) | 185-96 |
| Number of pages | 12 |
| Journal | Biochemistry international |
| Volume | 15 |
| Issue number | 1 |
| Publication status | Published - Jul 1987 |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 6 Clean Water and Sanitation
Keywords
- Animals
- Binding Sites
- Cattle
- Chromatography, High Pressure Liquid
- Coenzymes
- Metalloproteins
- Milk
- Oxidation-Reduction
- Pteridines
- Spectrometry, Fluorescence
- Sulfhydryl Compounds
- Time Factors
- Xanthine Oxidase
- Journal Article
- Research Support, Non-U.S. Gov't
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